FastQCFastQC Report
Wed 4 Mar 2015
trimmedLRindole6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenametrimmedLRindole6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18146975
Sequences flagged as poor quality0
Sequence length36-50
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCAACGTTAGGTATGGAAGTGGAATTGTTACTTCAACCTTATACGTCCG746320.41126413630921954No Hit
GCACTTTTCAGGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCC705570.38880860308674037No Hit
CCCGTGTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACT698730.38503937984154385No Hit
GTGGGTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCG656450.3617407308931654No Hit
CTTCAATTTGCTATCCAAAGCTCCCCTACCGTAGTTTGGGTTTACCGCTT598610.32986765011799485No Hit
CCGCGTTCCACCAATATCGTTTCCAATATTGTATCGTCACTGTGGCACTT454360.2503778177905684No Hit
GCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCGAAGCCGA423110.23315731685308433No Hit
GCAACGTTAGGTATGGAAGTGGAATTGTTACTTCAACCTTATACGTCCGG399770.22029566911289622No Hit
GTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTATTTA306990.1691686906495435No Hit
GTTTTGTCGTAATATAATTATCAGGCATAATTATATCGAGGTAATCATCT305930.16858457125774406No Hit
CCGTGTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTA278050.15322112914135827No Hit
CTTCAATCGGCGTATCGCCACTCCGCATCATGATAACATGACCGATTGTA273310.1506091235591607No Hit
AGCAAGTCTACCGACGACTGGCAGATCCTTTAATACCCGTTTACCATGGG262730.14477895076176606No Hit
CTTTAATTCATCATCTTGTCCATCTGTTCCATCATTATCAGATGGGATGA256910.14157180466716904No Hit
CAAAAACCAATTACGATAGCACATACATTAATCCATGCGAATGTCTTTTG249760.1376317540526727No Hit
CTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCGCCGTCACCATAAAAT248920.13716886698747313No Hit
GTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCGAAGC240510.1325344857751774No Hit
GTTCTATTCAGCAAGTCTACCGACGACTGGCAGATCCTTTAATACCCGTT225370.12419149748098514No Hit
CGGACAGTTGCTGAACTGACCTTAAAAGGTAACTTTTCTGCTAAATGTTT224810.12388290610418543No Hit
GTTTGTGGGTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTT219790.12111660483358797No Hit
CGTGTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTAT212400.11704430077189172No Hit
GGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCGCCGTCACCA211210.11638854409619234No Hit
CCGCAACGTTAGGTATGGAAGTGGAATTGTTACTTCAACCTTATACGTCC210270.11587055142799282No Hit
GCTGGAGCAAGCTTTTGTCCGTTTTCTAAAGTACGACCTTCTCCAACGGC201400.11098268444189735No Hit
GTCGTAATATAATTATCAGGCATAATTATATCGAGGTAATCATCTATCTC200380.11042060729129785No Hit
CAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTATTTAGAT197220.10867927023649948No Hit
GCATAATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATACTCTC195110.10751654201320054No Hit
GAAACTTTGACCGATGGGGCTGTGAAGCCAATCGTTAACTGGAATCGTCC191880.10573663103630218No Hit
CACTTTTCAGGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCG190420.10493208923250295No Hit
GTGACTTTTCAAGCAAAACGTCTGTCAAGTCCTGGCCGGCCAAGTTTCCA185560.10225395692670541No Hit
GTCAAATGGTATTACATCATACCGCCCATGCCGCCTTGTGGAGCAGCAGG184950.10191781274840574No Hit
GGTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCGAAG184650.10175249593940588No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTGT84950.036.1216471
GTTCTAT37500.031.6072181
GACTCCG20150.031.1540683
CGCAACG116250.030.5309891
GCGACTC21850.029.3329121
CCGTGTC110300.027.8198932
GGAGTCC20750.027.39114435
TCTATTC39950.026.4821973
CGACTCC24650.025.8228912
ACTCTAG7450.025.33832715
CGAAGCC78900.025.04949842
GAGTCCG22850.024.99149336
CGCGGGG15900.024.8484429
TCGGCGT53700.024.1975387
TACGTGA29450.023.65173539
TCGCGGG16850.023.5777558
AATCGGC56150.023.5326335
CTACACA7850.023.2077059
ATCGGCG56400.023.1558976
GTCGGCT17300.023.0913351
Produced by FastQC (version 0.11.2)