FastQCFastQC Report
Wed 4 Mar 2015
trimmedLRindole5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenametrimmedLRindole5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18468709
Sequences flagged as poor quality0
Sequence length36-50
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTTTTCAGGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCC1002990.5430753172839531No Hit
CCCGTGTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACT824920.44665818276740404No Hit
CTTCAATTTGCTATCCAAAGCTCCCCTACCGTAGTTTGGGTTTACCGCTT734170.3975210178469973No Hit
GTGGGTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCG700460.3792685238583812No Hit
CGCAACGTTAGGTATGGAAGTGGAATTGTTACTTCAACCTTATACGTCCG691010.3741517612303058No Hit
GCAACGTTAGGTATGGAAGTGGAATTGTTACTTCAACCTTATACGTCCGG535390.2898903220577031No Hit
CCGCGTTCCACCAATATCGTTTCCAATATTGTATCGTCACTGTGGCACTT450650.2440073098774798No Hit
GTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTATTTA422580.2288086297748262No Hit
GCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCGAAGCCGA416900.2257331576343533No Hit
GTTTTGTCGTAATATAATTATCAGGCATAATTATATCGAGGTAATCATCT392050.21227796701978463No Hit
CCGTGTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTA351960.1905709814367642No Hit
GTCGCGGGTACCCTGCATCTTCACAGGGATTTCAATTTCACCGAGTCTCT348990.18896285603936908No Hit
CTTTAATTCATCATCTTGTCCATCTGTTCCATCATTATCAGATGGGATGA289660.15683825003685964No Hit
GGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCGCCGTCACCA274370.1485593822502699No Hit
CTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCGCCGTCACCATAAAAT259640.14058373002682537No Hit
GTCGTAATATAATTATCAGGCATAATTATATCGAGGTAATCATCTATCTC251190.13600842376150926No Hit
GTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCGAAGC249970.1353478469989429No Hit
GTTTGTGGGTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTT247600.13406459541920338No Hit
AGCAAGTCTACCGACGACTGGCAGATCCTTTAATACCCGTTTACCATGGG244130.13218574184042858No Hit
CGTGTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTAT240560.13025274262537787No Hit
CAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTATTTAGAT237010.12833057253758234No Hit
GCATAATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATACTCTC236150.12786492006560934No Hit
CTTCAATCGGCGTATCGCCACTCCGCATCATGATAACATGACCGATTGTA235980.12777287248394026No Hit
GTTCTATTCAGCAAGTCTACCGACGACTGGCAGATCCTTTAATACCCGTT229560.12429672263502553No Hit
GAAACTTTGACCGATGGGGCTGTGAAGCCAATCGTTAACTGGAATCGTCC227670.12327337010941046No Hit
GTCAAATGGTATTACATCATACCGCCCATGCCGCCTTGTGGAGCAGCAGG226470.1226236224740993No Hit
CGGACAGTTGCTGAACTGACCTTAAAAGGTAACTTTTCTGCTAAATGTTT224980.12181685249358794No Hit
GCTGGAGCAAGCTTTTGTCCGTTTTCTAAAGTACGACCTTCTCCAACGGC221690.12003546106010984No Hit
CACTTTTCAGGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCG215150.116494336447664No Hit
CAGGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCGCCGTCAC211880.11472377414144107No Hit
CCGCAACGTTAGGTATGGAAGTGGAATTGTTACTTCAACCTTATACGTCC207340.11226556225451384No Hit
CAATATTGTATCGTCACTGTGGCACTTTTCAGGAATACTAAGACATAGTT197470.10692138795407953No Hit
AGCTGGAGCAAGCTTTTGTCCGTTTTCTAAAGTACGACCTTCTCCAACGG194610.10537282275658792No Hit
AACAGCTGGAGCAAGCTTTTGTCCGTTTTCTAAAGTACGACCTTCTCCAA192670.10432239741283487No Hit
GGCATAATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATACTCT191210.10353187112320628No Hit
GTGACTTTTCAAGCAAAACGTCTGTCAAGTCCTGGCCGGCCAAGTTTCCA190790.10330445945084737No Hit
CAAAAACCAATTACGATAGCACATACATTAATCCATGCGAATGTCTTTTG189710.10271968657906733No Hit
GGTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCGAAG188790.10222154672532877No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTGT97550.036.7857441
GACTCCG22400.033.0194443
GTCGCGG61350.032.3759881
GAGTCTC58900.032.0009543
CGAGTCT61250.031.08071142
GCGACTC24750.030.6824421
GTCTCGG4450.030.5788171
GTTCTAT38000.030.4379961
CCGAGTC62750.030.33622641
CGCAACG109250.029.8328021
GGAGTCC18600.029.49378635
CACAGGG67150.028.69776722
TCACAGG67350.028.58011221
CGACTCC26050.028.56152
ACCGAGT67000.028.2928840
CGAAGCC73800.028.1156842
GTCGGCT20200.028.0322131
TCTATTC38650.027.5978093
CGCGGGG15100.027.1801919
CGGGTAC72700.026.626995
Produced by FastQC (version 0.11.2)