FastQCFastQC Report
Wed 4 Mar 2015
trimmedLRindole3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenametrimmedLRindole3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14747813
Sequences flagged as poor quality0
Sequence length36-50
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTGTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACT552720.37478099295129386No Hit
CTTCAATTTGCTATCCAAAGCTCCCCTACCGTAGTTTGGGTTTACCGCTT463780.3144737460394975No Hit
GCACTTTTCAGGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCC445020.30175321588360254No Hit
GTGGGTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCG434240.29444365751043905No Hit
GCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCGAAGCCGA420930.2854185905394922No Hit
CGCAACGTTAGGTATGGAAGTGGAATTGTTACTTCAACCTTATACGTCCG404780.2744678143125357No Hit
GCAACGTTAGGTATGGAAGTGGAATTGTTACTTCAACCTTATACGTCCGG350190.2374521564655044No Hit
AGCAAGTCTACCGACGACTGGCAGATCCTTTAATACCCGTTTACCATGGG306520.20784098632115827No Hit
GTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTATTTA299550.20311486184426125No Hit
GTCAAATGGTATTACATCATACCGCCCATGCCGCCTTGTGGAGCAGCAGG255310.17311719371543427No Hit
CCGCGTTCCACCAATATCGTTTCCAATATTGTATCGTCACTGTGGCACTT254820.17278494106210868No Hit
CCGTGTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTA242210.16423452073876987No Hit
GCTGGAGCAAGCTTTTGTCCGTTTTCTAAAGTACGACCTTCTCCAACGGC241420.16369884809361226No Hit
GTTCTATTCAGCAAGTCTACCGACGACTGGCAGATCCTTTAATACCCGTT237730.16119678219407854No Hit
CTTTAATTCATCATCTTGTCCATCTGTTCCATCATTATCAGATGGGATGA224470.15220561855510373No Hit
CTTCAATCGGCGTATCGCCACTCCGCATCATGATAACATGACCGATTGTA211670.14352636557027132No Hit
CAAAAACCAATTACGATAGCACATACATTAATCCATGCGAATGTCTTTTG204090.1383866204433159No Hit
GTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCGAAGC193610.1312804820619844No Hit
GTTTTGTCGTAATATAATTATCAGGCATAATTATATCGAGGTAATCATCT192200.13032440810037393No Hit
CAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTATTTAGAT181040.12275718440422319No Hit
AGCTGGAGCAAGCTTTTGTCCGTTTTCTAAAGTACGACCTTCTCCAACGG179820.12192994310410636No Hit
CTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCGCCGTCACCATAAAAT174880.11858029390527258No Hit
CGTGTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTAT169870.11518318004167805No Hit
GGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCGCCGTCACCA167770.11375924009885399No Hit
GCATAATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATACTCTC163560.11090457954681145No Hit
ATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATACTCTCCCCTG154800.10496471578531677No Hit
AACAGCTGGAGCAAGCTTTTGTCCGTTTTCTAAAGTACGACCTTCTCCAA148690.1008217286183382No Hit
GTGACTTTTCAAGCAAAACGTCTGTCAAGTCCTGGCCGGCCAAGTTTCCA147790.10021146864284217No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTGT69050.036.7152671
GTTCTAT35500.036.5415951
TCTATTC40800.030.6650073
CGCAACG66450.029.4974771
GGAGTCC20750.028.9835335
CGAAGCC68250.028.92429742
GACTCCG9300.027.8501453
GAGTCCG22200.027.3106736
CCGTGTC93700.027.10332
GGGTGTG9500.026.67680242
ACTCTAG6550.025.46868715
TTCTATT51400.025.3232782
GAAGCCG78550.025.20129643
AGGAGTC24650.024.64948734
GTCGGCT15200.024.4045891
GTATGAG24750.024.38870814
GCGACTC11150.024.213561
CTACACA7000.023.8311719
GTGGGTT85350.023.6598871
ATCCTAC7350.023.2936276
Produced by FastQC (version 0.11.2)