FastQCFastQC Report
Wed 4 Mar 2015
trimmedLRindole2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenametrimmedLRindole2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13198545
Sequences flagged as poor quality0
Sequence length36-50
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTGTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACT816290.6184696873784193No Hit
GTGGGTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCG654660.4960092191980252No Hit
CTTCAATTTGCTATCCAAAGCTCCCCTACCGTAGTTTGGGTTTACCGCTT582960.4416850493747606No Hit
CGCAACGTTAGGTATGGAAGTGGAATTGTTACTTCAACCTTATACGTCCG533000.4038323921311023No Hit
GCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCGAAGCCGA518530.3928690624610516No Hit
GCACTTTTCAGGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCC467550.35424359275965644No Hit
GCAACGTTAGGTATGGAAGTGGAATTGTTACTTCAACCTTATACGTCCGG447570.3391055604992823No Hit
GTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTATTTA386510.2928428853331939No Hit
CCGTGTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTA363640.27551521777589877No Hit
CCGCGTTCCACCAATATCGTTTCCAATATTGTATCGTCACTGTGGCACTT346240.26233194643803537No Hit
AGCAAGTCTACCGACGACTGGCAGATCCTTTAATACCCGTTTACCATGGG312310.23662456732920184No Hit
GTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCGAAGC267020.2023101788871425No Hit
GTTCTATTCAGCAAGTCTACCGACGACTGGCAGATCCTTTAATACCCGTT266690.20206015132728647No Hit
CTTTAATTCATCATCTTGTCCATCTGTTCCATCATTATCAGATGGGATGA258280.19568823684731917No Hit
CGTGTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTAT238600.1807775023686323No Hit
CAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTATTTAGAT233710.1770725485271293No Hit
GTCAAATGGTATTACATCATACCGCCCATGCCGCCTTGTGGAGCAGCAGG209670.1588584196212537No Hit
GTTTGTGGGTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTT202990.15379725568234984No Hit
GGTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCGAAG198460.1503650591788716No Hit
GTTTTGTCGTAATATAATTATCAGGCATAATTATATCGAGGTAATCATCT197790.14985742746643665No Hit
CAAAAACCAATTACGATAGCACATACATTAATCCATGCGAATGTCTTTTG189070.14325063861205914No Hit
CTTCAATCGGCGTATCGCCACTCCGCATCATGATAACATGACCGATTGTA184090.13947749543604995No Hit
GCATAATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATACTCTC166940.12648363891625933No Hit
AAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTATTTAGATT162960.12346815501254116No Hit
CAATATTGTATCGTCACTGTGGCACTTTTCAGGAATACTAAGACATAGTT162510.1231272083400102No Hit
GCTGGAGCAAGCTTTTGTCCGTTTTCTAAAGTACGACCTTCTCCAACGGC161800.1225892702566836No Hit
GGGTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCGAA158340.11996776917455675No Hit
ATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATACTCTCCCCTG149850.11353524195280616No Hit
CCGCAACGTTAGGTATGGAAGTGGAATTGTTACTTCAACCTTATACGTCC146770.11120165139414989No Hit
CTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCGCCGTCACCATAAAAT145500.11023942411834031No Hit
GGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCGCCGTCACCA144920.10979998174041154No Hit
GTTGCCATGCTGATATTGGCAACTGAGTCTTTAAGATCATCATAAATCAT142850.10823162704676917No Hit
GCCGCATCATGGACGAACCCGGAATGGATCTACGCGCGCTACTTGTTAGG141960.10755731029443018No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTGT95750.038.1834371
GTTCTAT36200.037.343151
TCTATTC37650.033.9814873
GGAGTCC12300.028.84155335
CGAAGCC87200.028.70726842
GACTCCG11300.027.7713133
CGCAACG90650.027.4284271
CCGTGTC136750.026.831672
AAGCCGA93700.026.43941544
TTCTATT52200.026.0651362
TACGTGA22400.025.87621139
GAGTCCG13900.025.86230936
GTCGGCT17850.025.8178391
GAAGCCG97850.025.48012243
AATCGGC33450.023.946145
GTGGGTT129850.023.8971581
GCGACTC13800.023.6944141
CGGGTCC2650.023.1873422
CGCGGGG5600.022.7290029
CAATCGG37350.022.6796044
Produced by FastQC (version 0.11.2)