FastQCFastQC Report
Wed 4 Mar 2015
trimmedLRindole1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenametrimmedLRindole1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15851242
Sequences flagged as poor quality0
Sequence length36-50
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTGTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACT929660.586490320443029No Hit
CGCAACGTTAGGTATGGAAGTGGAATTGTTACTTCAACCTTATACGTCCG775510.48924242024694337No Hit
GTGGGTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCG756470.4772307431808813No Hit
CTTCAATTTGCTATCCAAAGCTCCCCTACCGTAGTTTGGGTTTACCGCTT680030.429007392606838No Hit
GCACTTTTCAGGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCC627420.395817564327136No Hit
GCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCGAAGCCGA614300.3875406103824546No Hit
CCGCGTTCCACCAATATCGTTTCCAATATTGTATCGTCACTGTGGCACTT522790.3298101183490858No Hit
GCAACGTTAGGTATGGAAGTGGAATTGTTACTTCAACCTTATACGTCCGG467050.29464568139203223No Hit
GTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTATTTA413310.26074297521922885No Hit
CCGTGTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTA411900.25985345501633245No Hit
GTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCGAAGC306420.19330977345497596No Hit
CGTGTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTAT296700.1871777618435199No Hit
AGCAAGTCTACCGACGACTGGCAGATCCTTTAATACCCGTTTACCATGGG296210.18686863780137858No Hit
CAAAAACCAATTACGATAGCACATACATTAATCCATGCGAATGTCTTTTG287460.1813485656202839No Hit
CAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTATTTAGAT271960.1715701520423447No Hit
CTTTAATTCATCATCTTGTCCATCTGTTCCATCATTATCAGATGGGATGA270370.17056707606886579No Hit
CTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCGCCGTCACCATAAAAT257600.16251092501142814No Hit
GTCAAATGGTATTACATCATACCGCCCATGCCGCCTTGTGGAGCAGCAGG233990.1476161931033543No Hit
GTTTTGTCGTAATATAATTATCAGGCATAATTATATCGAGGTAATCATCT230160.14519997865151513No Hit
GTTCTATTCAGCAAGTCTACCGACGACTGGCAGATCCTTTAATACCCGTT227580.14357234593983234No Hit
CTTCAATCGGCGTATCGCCACTCCGCATCATGATAACATGACCGATTGTA226400.14282792477712472No Hit
GTTTGTGGGTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTT226150.14267020842909345No Hit
CAATATTGTATCGTCACTGTGGCACTTTTCAGGAATACTAAGACATAGTT221530.13975561031747544No Hit
CGGACAGTTGCTGAACTGACCTTAAAAGGTAACTTTTCTGCTAAATGTTT218490.1378377795254151No Hit
GGTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCGAAG217230.13704288913133747No Hit
GGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCGCCGTCACCA216750.13674007374311742No Hit
GCCGCATCATGGACGAACCCGGAATGGATCTACGCGCGCTACTTGTTAGG198110.12498074283390538No Hit
CCGCAACGTTAGGTATGGAAGTGGAATTGTTACTTCAACCTTATACGTCC195940.12361176493299389No Hit
GCTGGAGCAAGCTTTTGTCCGTTTTCTAAAGTACGACCTTCTCCAACGGC191820.12101259951743844No Hit
GCATAATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATACTCTC186410.11759961774604161No Hit
CACTTTTCAGGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCG183860.11599091099612256No Hit
ATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATACTCTCCCCTG180090.11361254846781091No Hit
AAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTATTTAGATT176390.11127834651694801No Hit
GGGTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCGAA176250.11119002536205048No Hit
CAGGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCGCCGTCAC173190.10925957726214766No Hit
GTTGCCATGCTGATATTGGCAACTGAGTCTTTAAGATCATCATAAATCAT171160.1079789205161337No Hit
CGCCATTTCAATCTCTACGTTCATATCTCAACTATTTAGATTTCACTAAC168620.1063765224201359No Hit
AGCTGGAGCAAGCTTTTGTCCGTTTTCTAAAGTACGACCTTCTCCAACGG166000.10472365509276813No Hit
GTAAAATTCACGTTTACGATATTCTTGCAATGTTCCGTTACGTGAAACTG163650.1032411214212741No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTGT112250.037.8775021
GTTCTAT32750.034.0479131
CGCAACG118500.029.8411311
TCTATTC35850.029.0831323
GACTCCG14200.029.060713
CCGTGTC149950.028.4569192
CGAAGCC105900.027.24466142
GGAGTCC14600.027.00633435
GTCGGCT17500.026.5910741
AAGCCGA109100.026.23046144
GCGACTC16250.026.070061
AATCGGC45250.025.758115
CAATCGG51150.024.3747084
TCGGCGT47400.023.9414427
GTGGGTT154100.023.8161581
TACGTGA32850.023.5589839
GAAGCCG122850.023.48361643
TTCTATT48450.023.4678922
GAGTCCG17300.023.06708336
CCTATGC5150.022.5894363
Produced by FastQC (version 0.11.2)