FastQCFastQC Report
Wed 4 Mar 2015
trimmedLRehec7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenametrimmedLRehec7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19108243
Sequences flagged as poor quality0
Sequence length36-50
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTGTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACT1823480.9542897272135382No Hit
CTTCAATTTGCTATCCAAAGCTCCCCTACCGTAGTTTGGGTTTACCGCTT1684180.8813892517485779No Hit
CGCAACGTTAGGTATGGAAGTGGAATTGTTACTTCAACCTTATACGTCCG1245940.6520432046002346No Hit
GCACTTTTCAGGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCC1052280.5506942736702689No Hit
CCGCGTTCCACCAATATCGTTTCCAATATTGTATCGTCACTGTGGCACTT1015610.5315036029215245No Hit
CTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCGCCGTCACCATAAAAT870130.4553689211509399No Hit
GTGGGTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCG680600.3561813611015937No Hit
GTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTATTTA640650.33527415367284163No Hit
CCGTGTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTA583530.30538129539173225No Hit
CAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTATTTAGAT535660.280329279881986No Hit
CAATATTGTATCGTCACTGTGGCACTTTTCAGGAATACTAAGACATAGTT509710.2667487534044862No Hit
CGTGTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTAT487880.25532436446406925No Hit
CAGGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCGCCGTCAC462590.24208923865998563No Hit
GGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCGCCGTCACCA454660.23793919723545487No Hit
CACTTTTCAGGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCG452200.23665179472544912No Hit
GTTTTGTCGTAATATAATTATCAGGCATAATTATATCGAGGTAATCATCT441240.2309160502093259No Hit
CACCAATATCGTTTCCAATATTGTATCGTCACTGTGGCACTTTTCAGGAA411590.21539918662328086No Hit
CTTTAATTCATCATCTTGTCCATCTGTTCCATCATTATCAGATGGGATGA365280.1911635727052456No Hit
CGCCATTTCAATCTCTACGTTCATATCTCAACTATTTAGATTTCACTAAC365220.19113217264402593No Hit
ATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATACTCTCCCCTG358830.18778806612413293No Hit
GCATAATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATACTCTC327090.17117743373893665No Hit
CTTTTCAGGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCGCC325740.17047093236149446No Hit
GTCGTAATATAATTATCAGGCATAATTATATCGAGGTAATCATCTATCTC314180.1644211872331747No Hit
CAATTTGCTATCCAAAGCTCCCCTACCGTAGTTTGGGTTTACCGCTTGAG306260.16027637915218054No Hit
GCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCGAAGCCGA302330.15821967514229332No Hit
CTTGTCCAAGTGCAGGACCTACTGGTGGAGCTGGTGTAGCGGCACCGGCA302250.15817780839400042No Hit
GGCATAATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATACTCT293300.15349396592873557No Hit
ATCAGGCATAATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATA292490.15307006510227025No Hit
AAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTATTTAGATT288890.1511860614290911No Hit
GTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCGAAGC286680.15002949250750056No Hit
CATAATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATACTCTCC281760.14745468748748905No Hit
ATCGAGGTAATCATCTATCTCAGAGTATCGAAATACTCTCCCCTGGAATC278450.14572245077687154No Hit
CAAAAACCAATTACGATAGCACATACATTAATCCATGCGAATGTCTTTTG259880.136004131829389No Hit
GCAACGTTAGGTATGGAAGTGGAATTGTTACTTCAACCTTATACGTCCGG256760.1343713286459671No Hit
CAGCAATCTTGTCATGAGTACCTTCAATTGAAGGATCCATTGAAATGCGA243820.12759938210959532No Hit
AGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTATTTAGATTT243070.12720688134434968No Hit
GTTTTCGCGAGCAGCTTCAGCCAAAGCTTGAACACGTCCATGGTAAAGGT242770.1270498810382514No Hit
CCAATATTGTATCGTCACTGTGGCACTTTTCAGGAATACTAAGACATAGT241010.12612881257580824No Hit
CCGCAACGTTAGGTATGGAAGTGGAATTGTTACTTCAACCTTATACGTCC223760.11710129497515812No Hit
GGCACTTTTCAGGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTC216450.11327572084989708No Hit
CGCGTTCCACCAATATCGTTTCCAATATTGTATCGTCACTGTGGCACTTT207870.10878551209548674No Hit
CAGGCATAATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATACT205050.10730970921816307No Hit
GTCACTGTGGCACTTTTCAGGAATACTAAGACATAGTTAAAACCTTAGTC197970.10360450199424405No Hit
GGTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCGAAG195760.10244793307265351No Hit
GTAAAATTCACGTTTACGATATTCTTGCAATGTTCCGTTACGTGAAACTG194610.10184609856594351No Hit
CGTCACTGTGGCACTTTTCAGGAATACTAAGACATAGTTAAAACCTTAGT193090.10105063034837897No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTGT205200.040.894441
CGCAACG176850.032.8290251
CGGGTCC3250.031.0678962
CCGTGTC281050.029.974972
CCTATCT23950.029.2363073
CTATCTA25050.028.3029794
TCCTATC28050.027.9365442
CGTTAGG211300.027.5182616
TAGGTAT218250.026.641969
ACGTTAG229350.025.9937845
AGGTATG224150.025.96028510
TTAGGTA225850.025.8426328
CCTATGC4600.025.767563
GTATGGA226250.025.71953212
TATGGAA227050.025.70638713
GGTATGG226150.025.69187711
GTTAGGT231950.025.0967587
ATGGAAG234100.025.02612514
GTGTCCA344400.024.7098454
CCTCTCG4450.024.6630823
Produced by FastQC (version 0.11.2)