FastQCFastQC Report
Wed 4 Mar 2015
trimmedLRehec6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenametrimmedLRehec6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20084358
Sequences flagged as poor quality0
Sequence length36-50
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTGTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACT1511550.752600605904356No Hit
CTTCAATTTGCTATCCAAAGCTCCCCTACCGTAGTTTGGGTTTACCGCTT1369320.6817843019926253No Hit
GCACTTTTCAGGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCC1252290.6235150757619436No Hit
CGCAACGTTAGGTATGGAAGTGGAATTGTTACTTCAACCTTATACGTCCG1215810.6053516871189012No Hit
CCGCGTTCCACCAATATCGTTTCCAATATTGTATCGTCACTGTGGCACTT1141290.5682481859763703No Hit
CTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCGCCGTCACCATAAAAT678310.33773048658065147No Hit
GTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTATTTA654850.32604975473948433No Hit
GTGGGTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCG632400.3148719018053751No Hit
CCGTGTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTA615890.30665157432465606No Hit
GCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCGAAGCCGA612090.30475955467433913No Hit
GCAACGTTAGGTATGGAAGTGGAATTGTTACTTCAACCTTATACGTCCGG539470.2686020633569667No Hit
CAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTATTTAGAT517060.2574441264191765No Hit
CGTGTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTAT484000.24098355546141925No Hit
GGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCGCCGTCACCA470810.23441625567518762No Hit
GTTTTGTCGTAATATAATTATCAGGCATAATTATATCGAGGTAATCATCT450760.22443336252022597No Hit
CGCCATTTCAATCTCTACGTTCATATCTCAACTATTTAGATTTCACTAAC398560.19844298732376708No Hit
CAGGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCGCCGTCAC397860.19809445738818238No Hit
CACTTTTCAGGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCG386190.19228396546207752No Hit
ATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATACTCTCCCCTG361740.180110312712012No Hit
CAATATTGTATCGTCACTGTGGCACTTTTCAGGAATACTAAGACATAGTT361000.17974186678010817No Hit
GCATAATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATACTCTC345350.1719497332202503No Hit
GTGACGTCCCCCTTCTTCCTTAGTCATAACGTAAACTTCACCCTTGAAGT339540.1690569347548973No Hit
AAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTATTTAGATT323050.16084656527233782No Hit
CACCAATATCGTTTCCAATATTGTATCGTCACTGTGGCACTTTTCAGGAA317010.15783924982814984No Hit
TTGCTATCCAAAGCTCCCCTACCGTAGTTTGGGTTTACCGCTTGAGGGGT314590.15663433205084276No Hit
GTCGTAATATAATTATCAGGCATAATTATATCGAGGTAATCATCTATCTC289900.1443411833228625No Hit
GTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCGAAGC280790.13980531516118164No Hit
CCGCAACGTTAGGTATGGAAGTGGAATTGTTACTTCAACCTTATACGTCC280200.13951155421547456No Hit
CAATTTGCTATCCAAAGCTCCCCTACCGTAGTTTGGGTTTACCGCTTGAG279810.1393173732513631No Hit
GTTTTCGCGAGCAGCTTCAGCCAAAGCTTGAACACGTCCATGGTAAAGGT276830.13783363152558822No Hit
GGCATAATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATACTCT276560.13769919855043414No Hit
CAAAAACCAATTACGATAGCACATACATTAATCCATGCGAATGTCTTTTG273400.1361258348412232No Hit
ATCGAGGTAATCATCTATCTCAGAGTATCGAAATACTCTCCCCTGGAATC262650.1307734108304582No Hit
CTTTTCAGGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCGCC257730.12832374328320578No Hit
CGCGTTCCACCAATATCGTTTCCAATATTGTATCGTCACTGTGGCACTTT257590.12825403729608884No Hit
AGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTATTTAGATTT252980.12595871872030961No Hit
CATAATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATACTCTCC250670.12480856993288011No Hit
ATCAGGCATAATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATA244010.1214925565457457No Hit
TAATTATCAGGCATAATTATATCGAGGTAATCATCTATCTCAGAGTATCG240000.11949597791475336No Hit
GCTATCCAAAGCTCCCCTACCGTAGTTTGGGTTTACCGCTTGAGGGGTTT228180.11361080100245174No Hit
CAGATTTGAAATTTGACTAGCTCCGCGGAAATTACCGCCACATCCTGTGG217820.10845255795579824No Hit
CTTTAATTCATCATCTTGTCCATCTGTTCCATCATTATCAGATGGGATGA212120.10561452848032284No Hit
TGCTATCCAAAGCTCCCCTACCGTAGTTTGGGTTTACCGCTTGAGGGGTT205420.10227859909686932No Hit
CTTAGCCTTACGTAACCACTTCATGTGTTGAGTAAGCATTGAATCAACAA203820.1014819592441043No Hit
GCCGCATCATGGACGAACCCGGAATGGATCTACGCGCGCTACTTGTTAGG202780.10096414333980702No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTGT168500.040.736761
CGGGTCC2500.035.1219372
CGCAACG167650.032.4456331
CCTATGC6700.030.141963
TCCTATG6900.029.268282
AAGCCGA108200.028.67477444
CCGTGTC242050.028.3402332
GGAGTCC9650.025.34301835
GAAGCCG123650.025.18734743
CGAAGCC123450.025.12964842
GGGGGGT701.1556167E-625.08778421
CTATCTA24800.024.8721394
TGACGTC60500.024.7087172
TCCTATC25700.024.6844352
CCTATCT24050.024.5524983
CCGCGTT217200.023.5379241
GACTCCG5500.023.148553
CGTTAGG234500.023.1213196
CGTGTCC303750.022.7497633
GTGTCCA303150.022.7368644
Produced by FastQC (version 0.11.2)