FastQCFastQC Report
Wed 4 Mar 2015
trimmedLRehec5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenametrimmedLRehec5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15871869
Sequences flagged as poor quality0
Sequence length36-50
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTTTTCAGGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCC1207660.7608807759187024No Hit
CCCGTGTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACT1147650.7230717441027266No Hit
CTTCAATTTGCTATCCAAAGCTCCCCTACCGTAGTTTGGGTTTACCGCTT1087390.6851052009060811No Hit
CGCAACGTTAGGTATGGAAGTGGAATTGTTACTTCAACCTTATACGTCCG895870.5644388824025702No Hit
CCGCGTTCCACCAATATCGTTTCCAATATTGTATCGTCACTGTGGCACTT799170.5035134803594964No Hit
GTGGGTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCG599610.3777815958536452No Hit
GCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCGAAGCCGA583270.3674866520130679No Hit
GCAACGTTAGGTATGGAAGTGGAATTGTTACTTCAACCTTATACGTCCGG574900.3622131709882434No Hit
GTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTATTTA557790.3514330920952032No Hit
CTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCGCCGTCACCATAAAAT534040.33646951093157335No Hit
CCGTGTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTA506050.3188345367517839No Hit
GGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCGCCGTCACCA425690.26820407854928746No Hit
GTTTTGTCGTAATATAATTATCAGGCATAATTATATCGAGGTAATCATCT400920.25259785095252485No Hit
CAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTATTTAGAT392910.2475511863158649No Hit
CGTGTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTAT362520.2284041028816455No Hit
CAGGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCGCCGTCAC333900.21037220002256823No Hit
CACTTTTCAGGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCG327230.2061697963862983No Hit
GCATAATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATACTCTC319720.20143815451097788No Hit
CAATATTGTATCGTCACTGTGGCACTTTTCAGGAATACTAAGACATAGTT317550.20007095572676412No Hit
GTGACGTCCCCCTTCTTCCTTAGTCATAACGTAAACTTCACCCTTGAAGT307770.19390911051496204No Hit
ATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATACTCTCCCCTG288830.18197604831541894No Hit
AAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTATTTAGATT264680.16676044894271747No Hit
GTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCGAAGC261430.16471280099401023No Hit
GTCGTAATATAATTATCAGGCATAATTATATCGAGGTAATCATCTATCTC258560.16290457034392106No Hit
CACCAATATCGTTTCCAATATTGTATCGTCACTGTGGCACTTTTCAGGAA255370.16089472512657457No Hit
GGCATAATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATACTCT245180.154474561250474No Hit
CAAAAACCAATTACGATAGCACATACATTAATCCATGCGAATGTCTTTTG242080.15252142013016867No Hit
CGCCATTTCAATCTCTACGTTCATATCTCAACTATTTAGATTTCACTAAC238150.1500453412260396No Hit
CAATTTGCTATCCAAAGCTCCCCTACCGTAGTTTGGGTTTACCGCTTGAG225310.1419555567148393No Hit
CCGCAACGTTAGGTATGGAAGTGGAATTGTTACTTCAACCTTATACGTCC219290.13816268266831083No Hit
GTTTTCGCGAGCAGCTTCAGCCAAAGCTTGAACACGTCCATGGTAAAGGT218500.1376649467053943No Hit
CATAATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATACTCTCC211330.13314752030778482No Hit
GCTATCCAAAGCTCCCCTACCGTAGTTTGGGTTTACCGCTTGAGGGGTTT207850.13095496188886135No Hit
ATCGAGGTAATCATCTATCTCAGAGTATCGAAATACTCTCCCCTGGAATC204780.12902072213423638No Hit
GCCGCATCATGGACGAACCCGGAATGGATCTACGCGCGCTACTTGTTAGG203310.12809455521589802No Hit
ATCAGGCATAATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATA203240.12805045202931048No Hit
AGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTATTTAGATTT202930.12785513791727995No Hit
TAATTATCAGGCATAATTATATCGAGGTAATCATCTATCTCAGAGTATCG201740.12710538374529173No Hit
CTTTTCAGGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCGCC196590.12386064930349412No Hit
CGCGTTCCACCAATATCGTTTCCAATATTGTATCGTCACTGTGGCACTTT192160.12106954763802548No Hit
TTGCTATCCAAAGCTCCCCTACCGTAGTTTGGGTTTACCGCTTGAGGGGT189610.11946293155519366No Hit
TGCTATCCAAAGCTCCCCTACCGTAGTTTGGGTTTACCGCTTGAGGGGTT183660.11571416069525271No Hit
GGCACTTTTCAGGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTC181750.11451077374693554No Hit
GGTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCGAAG176960.1114928556933024No Hit
CAGATTTGAAATTTGACTAGCTCCGCGGAAATTACCGCCACATCCTGTGG172080.10841823354262815No Hit
CTTTAATTCATCATCTTGTCCATCTGTTCCATCATTATCAGATGGGATGA170650.10751726844519696No Hit
GTCACCATAAAATGGTGCCCCGCCTTATTTTTTCAGCGAGCACAAACACT164130.10340937163732891No Hit
GCCATTTCAATCTCTACGTTCATATCTCAACTATTTAGATTTCACTAACC162310.10226268878605285No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTGT126200.040.2266431
CGCAACG125750.031.3993531
AAGCCGA93650.030.41363144
CCTCGAG300.005813852429.264085
GAAGCCG101800.028.2273443
CGAAGCC103100.027.86175342
CCGTGTC184100.027.6348532
TGACGTC55550.026.1955182
TCCTATG4900.025.5314962
CCTATCT16950.025.5088753
GACTCCG5600.025.4753483
CTATCTA17600.025.1903084
GTGGGTT119250.024.8285331
CCTATGC5050.024.7729823
TCCTATC19350.024.6136652
TACGTGA29950.023.4101539
ACGTCCC62550.023.4041734
GCGACTC6850.022.4287451
CTTAGAC22150.022.3940281
GGAGTCC6900.022.3557335
Produced by FastQC (version 0.11.2)