FastQCFastQC Report
Wed 4 Mar 2015
trimmedLRehec4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenametrimmedLRehec4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19068866
Sequences flagged as poor quality0
Sequence length36-50
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCAATTTGCTATCCAAAGCTCCCCTACCGTAGTTTGGGTTTACCGCTT1708830.896136141498923No Hit
CCCGTGTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACT1677900.8799159845163316No Hit
GCACTTTTCAGGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCC1504520.7889929060280774No Hit
CCGCGTTCCACCAATATCGTTTCCAATATTGTATCGTCACTGTGGCACTT1354650.7103988249746996No Hit
CGCAACGTTAGGTATGGAAGTGGAATTGTTACTTCAACCTTATACGTCCG1134490.5949436112247053No Hit
GCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCGAAGCCGA928230.48677776643875936No Hit
CTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCGCCGTCACCATAAAAT801020.42006693004188084No Hit
GTGGGTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCG788520.413511742124571No Hit
GTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTATTTA748950.39276063925353505No Hit
CCGTGTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTA674320.3536235453120285No Hit
GTTTTGTCGTAATATAATTATCAGGCATAATTATATCGAGGTAATCATCT592430.31067919822814843No Hit
CAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTATTTAGAT583330.3059070214243469No Hit
GGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCGCCGTCACCA572820.30039541942347275No Hit
GCAACGTTAGGTATGGAAGTGGAATTGTTACTTCAACCTTATACGTCCGG569800.29881168602265074No Hit
CAATATTGTATCGTCACTGTGGCACTTTTCAGGAATACTAAGACATAGTT545270.28594778525372194No Hit
CGTGTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTAT534180.2801320225334847No Hit
CAGGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCGCCGTCAC473630.24837869226203593No Hit
GCATAATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATACTCTC468050.2454524563757488No Hit
ATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATACTCTCCCCTG451630.23684156152757063No Hit
CGCCATTTCAATCTCTACGTTCATATCTCAACTATTTAGATTTCACTAAC445720.23374226868026654No Hit
CACTTTTCAGGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCG438520.2299664804398961No Hit
CACCAATATCGTTTCCAATATTGTATCGTCACTGTGGCACTTTTCAGGAA417890.21914779830116798No Hit
GTGACGTCCCCCTTCTTCCTTAGTCATAACGTAAACTTCACCCTTGAAGT392390.20577521494985598No Hit
AAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTATTTAGATT377040.1977254441873995No Hit
GTCGTAATATAATTATCAGGCATAATTATATCGAGGTAATCATCTATCTC375440.19688638013398385No Hit
GGCATAATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATACTCT370650.19437443212407074No Hit
CAATTTGCTATCCAAAGCTCCCCTACCGTAGTTTGGGTTTACCGCTTGAG358120.18780351175575935No Hit
GTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCGAAGC356490.18694871525134216No Hit
TTGCTATCCAAAGCTCCCCTACCGTAGTTTGGGTTTACCGCTTGAGGGGT355210.18627746400860962No Hit
ATCGAGGTAATCATCTATCTCAGAGTATCGAAATACTCTCCCCTGGAATC338870.17770852236310225No Hit
CATAATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATACTCTCC335810.1761038123609448No Hit
GCTATCCAAAGCTCCCCTACCGTAGTTTGGGTTTACCGCTTGAGGGGTTT314760.16506487590819507No Hit
CAAAAACCAATTACGATAGCACATACATTAATCCATGCGAATGTCTTTTG314590.16497572535251964No Hit
GCCGCATCATGGACGAACCCGGAATGGATCTACGCGCGCTACTTGTTAGG311100.16314551688600676No Hit
TAATTATCAGGCATAATTATATCGAGGTAATCATCTATCTCAGAGTATCG305150.1600252474373673No Hit
CGCGTTCCACCAATATCGTTTCCAATATTGTATCGTCACTGTGGCACTTT298470.1565221550143569No Hit
ATCAGGCATAATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATA296540.15551003399992427No Hit
CTTTAATTCATCATCTTGTCCATCTGTTCCATCATTATCAGATGGGATGA294090.15422521716813153No Hit
AGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTATTTAGATTT294000.1541780198151269No Hit
CTTTTCAGGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCGCC291110.1526624603686449No Hit
TGCTATCCAAAGCTCCCCTACCGTAGTTTGGGTTTACCGCTTGAGGGGTT270180.14168645371990132No Hit
CCGCAACGTTAGGTATGGAAGTGGAATTGTTACTTCAACCTTATACGTCC261650.13721319348512911No Hit
GGTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCGAAG259700.1361905841700288No Hit
CCAATATTGTATCGTCACTGTGGCACTTTTCAGGAATACTAAGACATAGT253930.13316470942739855No Hit
GAAATACTCTCCCCTGGAATCACTTCAATTTGCTATCCAAAGCTCCCCTA249370.13077337687516397No Hit
GCCATTTCAATCTCTACGTTCATATCTCAACTATTTAGATTTCACTAACC241900.12685599657577962No Hit
GTCACTGTGGCACTTTTCAGGAATACTAAGACATAGTTAAAACCTTAGTC236800.1241814799055172No Hit
GGCACTTTTCAGGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTC236720.12413952670284642No Hit
CAGGCATAATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATACT236080.12380390108148015No Hit
CGTCACTGTGGCACTTTTCAGGAATACTAAGACATAGTTAAAACCTTAGT222110.11647782306509469No Hit
GTAAAATTCACGTTTACGATATTCTTGCAATGTTCCGTTACGTGAAACTG211790.1110658599205637No Hit
GTTTTCGCGAGCAGCTTCAGCCAAAGCTTGAACACGTCCATGGTAAAGGT204180.10707506151650548No Hit
GGGTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCGAA201280.10555425791968961No Hit
GTTAAAACCTTAGTCCGTTTTTCCGCCGTCACCATAAAATGGTGCCCCGC199340.10453689275492313No Hit
GTTTGTGGGTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTT199170.10444774219924773No Hit
TTCAGGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCGCCGTC191020.1001737596771617No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTGT192600.040.2020031
AAGCCGA138750.031.5997844
CGCAACG169750.031.1160411
CCGTGTC270150.028.734642
GAAGCCG158500.027.96247743
CGAAGCC159300.027.81869142
TGACGTC73700.026.0043052
ATCTAGG4100.025.1659031
GACTCCG5350.025.0307243
CCTATGC2750.024.7472173
GTGGGTT152800.024.108331
CCGCGTT266200.023.6191061
ACGTCCC81500.023.515494
TCTAGGT4450.023.1865622
CGTGTCC338800.023.0547243
TACGTGA40500.023.03046839
ACTCTAG5150.023.01922815
GTGTCCA343950.022.7414344
CGGGTCC3400.022.5989192
CTATCTA19850.022.561454
Produced by FastQC (version 0.11.2)