FastQCFastQC Report
Wed 4 Mar 2015
trimmedLRehec2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenametrimmedLRehec2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16070567
Sequences flagged as poor quality0
Sequence length36-50
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTGTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACT1925541.1981780107696263No Hit
GCACTTTTCAGGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCC1456590.9063712562226336No Hit
CTTCAATTTGCTATCCAAAGCTCCCCTACCGTAGTTTGGGTTTACCGCTT1417930.8823148554746076No Hit
CCGCGTTCCACCAATATCGTTTCCAATATTGTATCGTCACTGTGGCACTT1122120.69824543216179No Hit
CGCAACGTTAGGTATGGAAGTGGAATTGTTACTTCAACCTTATACGTCCG1038810.6464053197376297No Hit
GTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTATTTA812300.5054582081640305No Hit
CCGTGTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTA797770.49641683457714963No Hit
GTGGGTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCG708690.44098630745262446No Hit
CGTGTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTAT591200.3678774992817615No Hit
GTTTTGTCGTAATATAATTATCAGGCATAATTATATCGAGGTAATCATCT583030.36279367118783057No Hit
CAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTATTTAGAT571860.35584307635194207No Hit
CTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCGCCGTCACCATAAAAT551070.34290638283017644No Hit
GCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCGAAGCCGA489500.3045941067294017No Hit
GCAACGTTAGGTATGGAAGTGGAATTGTTACTTCAACCTTATACGTCCGG468260.29137739819634245No Hit
CACTTTTCAGGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCG432350.26903220029511093No Hit
GGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCGCCGTCACCA430110.2676383477944493No Hit
CAATATTGTATCGTCACTGTGGCACTTTTCAGGAATACTAAGACATAGTT398660.24806840978292802No Hit
GCATAATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATACTCTC395370.24602118892258126No Hit
CAGGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCGCCGTCAC394400.24541760100934834No Hit
GTCGTAATATAATTATCAGGCATAATTATATCGAGGTAATCATCTATCTC367970.22897138601270262No Hit
AAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTATTTAGATT357310.2223381415229469No Hit
CGCCATTTCAATCTCTACGTTCATATCTCAACTATTTAGATTTCACTAAC355880.22144831604261384No Hit
ATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATACTCTCCCCTG353670.22007313120937178No Hit
CACCAATATCGTTTCCAATATTGTATCGTCACTGTGGCACTTTTCAGGAA340190.21168512598217598No Hit
GTGACGTCCCCCTTCTTCCTTAGTCATAACGTAAACTTCACCCTTGAAGT325600.20260641706045593No Hit
GGCATAATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATACTCT312530.19447353662132766No Hit
TAATTATCAGGCATAATTATATCGAGGTAATCATCTATCTCAGAGTATCG287260.17874913809823884No Hit
CAATTTGCTATCCAAAGCTCCCCTACCGTAGTTTGGGTTTACCGCTTGAG284400.17696948713757268No Hit
CTTTAATTCATCATCTTGTCCATCTGTTCCATCATTATCAGATGGGATGA272680.16967665173232532No Hit
ATCAGGCATAATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATA272520.1695770908394209No Hit
CCGCAACGTTAGGTATGGAAGTGGAATTGTTACTTCAACCTTATACGTCC272320.16945263972329042No Hit
GTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCGAAGC269850.1679156684390787No Hit
TTGCTATCCAAAGCTCCCCTACCGTAGTTTGGGTTTACCGCTTGAGGGGT269310.16757965042552636No Hit
CTTTTCAGGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCGCC269310.16757965042552636No Hit
AGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTATTTAGATTT269010.1673929737513306No Hit
CATAATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATACTCTCC266470.16581244457647326No Hit
CGCGTTCCACCAATATCGTTTCCAATATTGTATCGTCACTGTGGCACTTT259550.1615064359583579No Hit
ATCGAGGTAATCATCTATCTCAGAGTATCGAAATACTCTCCCCTGGAATC247290.1538775825395582No Hit
CAAAAACCAATTACGATAGCACATACATTAATCCATGCGAATGTCTTTTG239720.14916710779401873No Hit
GCCGCATCATGGACGAACCCGGAATGGATCTACGCGCGCTACTTGTTAGG237360.1476985846236788No Hit
GGCACTTTTCAGGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTC237320.14767369440045272No Hit
GTTTGTGGGTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTT221070.13756204121484947No Hit
CAGGCATAATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATACT210340.1308852388344481No Hit
GAAATACTCTCCCCTGGAATCACTTCAATTTGCTATCCAAAGCTCCCCTA205820.12807264360989876No Hit
GTGTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTATT196390.12220477348434565No Hit
TGCTATCCAAAGCTCCCCTACCGTAGTTTGGGTTTACCGCTTGAGGGGTT195170.12144562167594958No Hit
CCAATATTGTATCGTCACTGTGGCACTTTTCAGGAATACTAAGACATAGT193670.12051223830497082No Hit
GCTATCCAAAGCTCCCCTACCGTAGTTTGGGTTTACCGCTTGAGGGGTTT192800.11997087594980314No Hit
GGTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCGAAG190770.11870769712107856No Hit
GCCATTTCAATCTCTACGTTCATATCTCAACTATTTAGATTTCACTAACC185060.11515461775555275No Hit
GTAAAATTCACGTTTACGATATTCTTGCAATGTTCCGTTACGTGAAACTG175660.10930541529741919No Hit
TCGTAATATAATTATCAGGCATAATTATATCGAGGTAATCATCTATCTCA174620.10865826949354059No Hit
CTGGAATCACTTCAATTTGCTATCCAAAGCTCCCCTACCGTAGTTTGGGT163590.10179479043894343No Hit
ATAATTATCAGGCATAATTATATCGAGGTAATCATCTATCTCAGAGTATC163420.10168900699023252No Hit
GGGTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCGAA162590.10117253485829093No Hit
GTTTTCGCGAGCAGCTTCAGCCAAAGCTTGAACACGTCCATGGTAAAGGT161480.10048183116376666No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTGT213550.040.5120621
CGGGTCC3650.035.4843332
CGCAACG156900.031.11641
AAGCCGA80450.029.23408944
CCGTGTC304950.028.463322
CGAAGCC91700.025.87898442
GAAGCCG91550.025.86829643
CCTATGC3800.025.4182243
GACTCCG6750.025.0416583
TACGTGA33050.024.88291539
CCTATCT16300.024.5109393
TCCTATG3950.024.453052
TGACGTC60650.024.3591292
CCGCGTT216550.023.5893341
GCGACTC7600.023.1075481
CGTGTCC377300.023.0691993
CTATCTA17700.023.0683144
GTGGGTT148300.023.0031551
CGTTAGG214400.022.9350326
GTGTCCA387150.022.4709264
Produced by FastQC (version 0.11.2)