FastQCFastQC Report
Wed 4 Mar 2015
trimmedLRcomm7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenametrimmedLRcomm7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14304301
Sequences flagged as poor quality0
Sequence length36-50
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTGTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACT1729191.208860188274841No Hit
GCACTTTTCAGGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCC1386690.9694217144899285No Hit
CTTCAATTTGCTATCCAAAGCTCCCCTACCGTAGTTTGGGTTTACCGCTT1159140.8103436861402734No Hit
CCGCGTTCCACCAATATCGTTTCCAATATTGTATCGTCACTGTGGCACTT815450.5700732947384147No Hit
CGCAACGTTAGGTATGGAAGTGGAATTGTTACTTCAACCTTATACGTCCG773180.5405227420759672No Hit
GTGGGTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCG736490.5148731140375191No Hit
CCGTGTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTA711470.49738187136861844No Hit
GTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTATTTA663140.4635948306736555No Hit
GTTTTGTCGTAATATAATTATCAGGCATAATTATATCGAGGTAATCATCT619340.43297466964656295No Hit
CGTGTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTAT477000.33346613721285645No Hit
GCAACGTTAGGTATGGAAGTGGAATTGTTACTTCAACCTTATACGTCCGG423990.2964073532848617No Hit
CAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTATTTAGAT402030.2810553273452509No Hit
GTCGTAATATAATTATCAGGCATAATTATATCGAGGTAATCATCTATCTC390220.27279906931488646No Hit
GTGACGTCCCCCTTCTTCCTTAGTCATAACGTAAACTTCACCCTTGAAGT372910.2606978138952753No Hit
CACTTTTCAGGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCG359340.2512111567003519No Hit
CAATATTGTATCGTCACTGTGGCACTTTTCAGGAATACTAAGACATAGTT344740.2410044363579877No Hit
GCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCGAAGCCGA333350.23304179631007485No Hit
GGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCGCCGTCACCA324880.22712050033063483No Hit
CTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCGCCGTCACCATAAAAT320070.2237578753411299No Hit
GCATAATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATACTCTC307470.21494933586758275No Hit
GTTTGTGGGTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTT303590.21223686498207778No Hit
CAGGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCGCCGTCAC295370.20649034161123989No Hit
CACCAATATCGTTTCCAATATTGTATCGTCACTGTGGCACTTTTCAGGAA283530.19821311086784318No Hit
GGCACTTTTCAGGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTC271430.18975411661150027No Hit
CTTTAATTCATCATCTTGTCCATCTGTTCCATCATTATCAGATGGGATGA268090.18741915456057587No Hit
GGCATAATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATACTCT252020.17618477127963122No Hit
CCGCAACGTTAGGTATGGAAGTGGAATTGTTACTTCAACCTTATACGTCC250870.1753808172800614No Hit
AAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTATTTAGATT244900.17120724738664267No Hit
TAATTATCAGGCATAATTATATCGAGGTAATCATCTATCTCAGAGTATCG243860.17048019333485784No Hit
ATCAGGCATAATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATA235170.1644050974598479No Hit
ATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATACTCTCCCCTG233860.16348928899077278No Hit
GTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCGAAGC226810.1585607014281928No Hit
CTTTTCAGGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCGCC213290.14910899875498984No Hit
CAATTTGCTATCCAAAGCTCCCCTACCGTAGTTTGGGTTTACCGCTTGAG201220.1406709772116792No Hit
GAAATACTCTCCCCTGGAATCACTTCAATTTGCTATCCAAAGCTCCCCTA200380.14008374124677606No Hit
CGCGTTCCACCAATATCGTTTCCAATATTGTATCGTCACTGTGGCACTTT197100.13779072462191616No Hit
GCCGCATCATGGACGAACCCGGAATGGATCTACGCGCGCTACTTGTTAGG193820.13549770799705627No Hit
AGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTATTTAGATTT188820.13200225582501374No Hit
CATAATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATACTCTCC188790.13198128311198148No Hit
CGCCATTTCAATCTCTACGTTCATATCTCAACTATTTAGATTTCACTAAC188540.13180651050337938No Hit
GGTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCGAAG185480.12966729377408934No Hit
AATCACTTCAATTTGCTATCCAAAGCTCCCCTACCGTAGTTTGGGTTTAC173810.1215089084045421No Hit
GTGTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTATT170430.11914598273624136No Hit
CAGGCATAATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATACT166930.1166991662158116No Hit
CCAATATTGTATCGTCACTGTGGCACTTTTCAGGAATACTAAGACATAGT166210.11619582110303746No Hit
GGGTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCGAA162490.11359520468703785No Hit
GTAAAATTCACGTTTACGATATTCTTGCAATGTTCCGTTACGTGAAACTG162230.11341344117409163No Hit
ATCGAGGTAATCATCTATCTCAGAGTATCGAAATACTCTCCCCTGGAATC152820.1068350001863076No Hit
ATCGTTTCCAATATTGTATCGTCACTGTGGCACTTTTCAGGAATACTAAG152610.10668819119508183No Hit
CAAAAACCAATTACGATAGCACATACATTAATCCATGCGAATGTCTTTTG150820.10543681931749059No Hit
TCGTAATATAATTATCAGGCATAATTATATCGAGGTAATCATCTATCTCA148530.10383590222269512No Hit
TTGCTATCCAAAGCTCCCCTACCGTAGTTTGGGTTTACCGCTTGAGGGGT147620.10319972992738338No Hit
CTGGAATCACTTCAATTTGCTATCCAAAGCTCCCCTACCGTAGTTTGGGT147110.10284319380583504No Hit
GTTTTCGCGAGCAGCTTCAGCCAAAGCTTGAACACGTCCATGGTAAAGGT145630.10180853996291045No Hit
ATAATTATCAGGCATAATTATATCGAGGTAATCATCTATCTCAGAGTATC143760.10050124085056655No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTGT192100.040.5451471
CGGGTCC3050.030.9493312
CGCAACG121850.029.3659951
AAGCCGA60400.029.04791344
CCGTGTC270300.028.8557132
GACTCCG8350.027.0789533
CGAAGCC68450.025.8929542
GAAGCCG68550.025.7631543
GCGACTC9250.025.3936211
GGAGTCC5550.025.00936735
CCTATGC3650.024.658813
TACGTGA27700.024.18191739
CCTATCT16250.024.1813643
CGTGTCC329700.023.8033563
GGGAGTC3150.023.6946892
TGACGTC71600.023.5160852
CTATCTA17450.023.3990734
GTGTCCA339200.023.1431664
ACTCTAG5300.022.78122315
CGTCCGG87000.022.60714344
Produced by FastQC (version 0.11.2)