FastQCFastQC Report
Wed 4 Mar 2015
trimmedLRcomm6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenametrimmedLRcomm6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20101189
Sequences flagged as poor quality0
Sequence length36-50
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTTTTCAGGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCC1695530.8434973672452908No Hit
CCCGTGTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACT1662340.8269859061570936No Hit
CTTCAATTTGCTATCCAAAGCTCCCCTACCGTAGTTTGGGTTTACCGCTT1434240.7135100316702659No Hit
CCGCGTTCCACCAATATCGTTTCCAATATTGTATCGTCACTGTGGCACTT1026010.510422542666506No Hit
GTGGGTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCG948750.4719870053458032No Hit
GCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCGAAGCCGA856880.42628324125503225No Hit
CGCAACGTTAGGTATGGAAGTGGAATTGTTACTTCAACCTTATACGTCCG822880.40936881892906934No Hit
GTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTATTTA804680.40031462815458324No Hit
CTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCGCCGTCACCATAAAAT709000.3527154537972853No Hit
CCGTGTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTA695970.34623325018236484No Hit
GTTTTGTCGTAATATAATTATCAGGCATAATTATATCGAGGTAATCATCT692770.34464130455168596No Hit
GGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCGCCGTCACCA617570.30723058223073274No Hit
GCAACGTTAGGTATGGAAGTGGAATTGTTACTTCAACCTTATACGTCCGG572130.2846249542750929No Hit
GTGACGTCCCCCTTCTTCCTTAGTCATAACGTAAACTTCACCCTTGAAGT549780.27350620901082023No Hit
CAATATTGTATCGTCACTGTGGCACTTTTCAGGAATACTAAGACATAGTT527760.2625516331397113No Hit
CGTGTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTAT511820.2546217539668922No Hit
CAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTATTTAGAT510680.2540546233359629No Hit
GCATAATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATACTCTC507050.25224876001116153No Hit
ATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATACTCTCCCCTG478700.238145116689366No Hit
CAGGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCGCCGTCAC450670.2242006679306383No Hit
CACTTTTCAGGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCG431260.21454452271455188No Hit
GTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCGAAGC410200.2040675305326466No Hit
GTCGTAATATAATTATCAGGCATAATTATATCGAGGTAATCATCTATCTC398890.198440997694216No Hit
GGCATAATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATACTCT380090.1890883171139777No Hit
GCCGCATCATGGACGAACCCGGAATGGATCTACGCGCGCTACTTGTTAGG374580.18634718573115253No Hit
CACCAATATCGTTTCCAATATTGTATCGTCACTGTGGCACTTTTCAGGAA355810.17700942964120184No Hit
AAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTATTTAGATT353500.17586024388905552No Hit
ATCGAGGTAATCATCTATCTCAGAGTATCGAAATACTCTCCCCTGGAATC339650.16897010420627356No Hit
CATAATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATACTCTCC336520.1674129823862658No Hit
CTTTAATTCATCATCTTGTCCATCTGTTCCATCATTATCAGATGGGATGA319960.1591746637475027No Hit
ATCAGGCATAATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATA319100.15874682835925774No Hit
GCTATCCAAAGCTCCCCTACCGTAGTTTGGGTTTACCGCTTGAGGGGTTT312180.15530424593291473No Hit
AGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTATTTAGATTT286620.1425885802078673No Hit
CGCCATTTCAATCTCTACGTTCATATCTCAACTATTTAGATTTCACTAAC283350.14096181076651734No Hit
GAAATACTCTCCCCTGGAATCACTTCAATTTGCTATCCAAAGCTCCCCTA282430.14050412639769716No Hit
TAATTATCAGGCATAATTATATCGAGGTAATCATCTATCTCAGAGTATCG281310.13994694542695957No Hit
GGTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCGAAG279010.13880273450490913No Hit
CAATTTGCTATCCAAAGCTCCCCTACCGTAGTTTGGGTTTACCGCTTGAG278920.1387579610340463No Hit
GGCACTTTTCAGGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTC268140.1333950941906969No Hit
GTTTGTGGGTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTT266070.1323653043608515No Hit
GTCACTGTGGCACTTTTCAGGAATACTAAGACATAGTTAAAACCTTAGTC266070.1323653043608515No Hit
GTAAAATTCACGTTTACGATATTCTTGCAATGTTCCGTTACGTGAAACTG253890.1263059613040801No Hit
CTTTTCAGGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCGCC245980.12237087069824576No Hit
CAAAAACCAATTACGATAGCACATACATTAATCCATGCGAATGTCTTTTG243230.12100279242188111No Hit
CGCGTTCCACCAATATCGTTTCCAATATTGTATCGTCACTGTGGCACTTT234130.1164756970346381No Hit
GGGTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCGAA231640.11523696434076611No Hit
GCCATTTCAATCTCTACGTTCATATCTCAACTATTTAGATTTCACTAACC226990.1129236683461859No Hit
TTGCTATCCAAAGCTCCCCTACCGTAGTTTGGGTTTACCGCTTGAGGGGT220130.10951093490041808No Hit
CCAATATTGTATCGTCACTGTGGCACTTTTCAGGAATACTAAGACATAGT217590.10824732805606674No Hit
CCGCAACGTTAGGTATGGAAGTGGAATTGTTACTTCAACCTTATACGTCC215700.10730708516794704No Hit
CAGGCATAATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATACT214810.10686432528941446No Hit
GTTAAAACCTTAGTCCGTTTTTCCGCCGTCACCATAAAATGGTGCCCCGC211150.10504353747432553No Hit
TGCTATCCAAAGCTCCCCTACCGTAGTTTGGGTTTACCGCTTGAGGGGTT207550.1032525986398118No Hit
CGTCACTGTGGCACTTTTCAGGAATACTAAGACATAGTTAAAACCTTAGT203180.10107859788791597No Hit
GTTTTCGCGAGCAGCTTCAGCCAAAGCTTGAACACGTCCATGGTAAAGGT201680.10033237337353526No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTGT195150.038.237131
AAGCCGA125400.032.43837444
CGGGTCC3700.030.8534892
CGAAGCC135200.030.4811842
GAAGCCG136850.030.0783243
GGCGGGT300.005806923429.271261
CGCAACG131400.028.6196751
CCGTGTC274700.027.2280272
TCCTATG4550.027.0196252
CCTATGC4500.026.8318563
GACTCCG10100.026.0831723
TGACGTC101100.025.7317832
TACGTGA47450.024.6882139
GTGGGTT178900.024.2481861
CTATCTA18700.023.9490954
ACGTCCC111700.023.3487594
CCTATCT18300.022.9130443
CCGAAGC180850.022.77269641
GCGACTC12600.021.9534451
CGTGTCC348000.021.6883813
Produced by FastQC (version 0.11.2)