FastQCFastQC Report
Wed 4 Mar 2015
trimmedLRcomm5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenametrimmedLRcomm5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15696217
Sequences flagged as poor quality0
Sequence length36-50
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCAATTTGCTATCCAAAGCTCCCCTACCGTAGTTTGGGTTTACCGCTT1622631.033771385805892No Hit
GCACTTTTCAGGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCC1535250.9781019209915358No Hit
CCCGTGTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACT1273370.8112591715570701No Hit
GTGGGTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCG870720.5547323918878032No Hit
GTTTTGTCGTAATATAATTATCAGGCATAATTATATCGAGGTAATCATCT750220.477962301362169No Hit
GTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTATTTA723570.4609836879803586No Hit
CTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCGCCGTCACCATAAAAT629520.4010647915991477No Hit
GGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCGCCGTCACCA622370.3965095538625645No Hit
CCGCGTTCCACCAATATCGTTTCCAATATTGTATCGTCACTGTGGCACTT618150.39382100795369995No Hit
CGCAACGTTAGGTATGGAAGTGGAATTGTTACTTCAACCTTATACGTCCG578130.36832441855257225No Hit
CAATATTGTATCGTCACTGTGGCACTTTTCAGGAATACTAAGACATAGTT528850.33692831846042903No Hit
GTCGTAATATAATTATCAGGCATAATTATATCGAGGTAATCATCTATCTC484620.30874955411230615No Hit
GCATAATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATACTCTC468300.29835214434153146No Hit
CAGGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCGCCGTCAC455200.29000618429268654No Hit
GGCATAATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATACTCT426690.2718425720031776No Hit
ATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATACTCTCCCCTG410780.2617063716690461No Hit
CCGTGTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTA387070.2466008210768238No Hit
CAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTATTTAGAT376610.2399367949614866No Hit
GTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCGAAGC363920.23185204434928494No Hit
CACCAATATCGTTTCCAATATTGTATCGTCACTGTGGCACTTTTCAGGAA350640.2233914069867918No Hit
GCAACGTTAGGTATGGAAGTGGAATTGTTACTTCAACCTTATACGTCCGG330270.21041375765893144No Hit
GCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCGAAGCCGA328950.20957279069217763No Hit
ATCAGGCATAATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATA326870.20824763062335339No Hit
CTTTAATTCATCATCTTGTCCATCTGTTCCATCATTATCAGATGGGATGA323970.2064000516812427No Hit
CACTTTTCAGGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCG320960.20448239215856917No Hit
CGTGTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTAT315660.20110578236781515No Hit
GGCACTTTTCAGGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTC314630.20044957329527235No Hit
AAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTATTTAGATT294060.1873445047300251No Hit
GTCACTGTGGCACTTTTCAGGAATACTAAGACATAGTTAAAACCTTAGTC292570.18639523141149233No Hit
GCTATCCAAAGCTCCCCTACCGTAGTTTGGGTTTACCGCTTGAGGGGTTT275080.175252419102004No Hit
GGTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCGAAG273190.17404830730869736No Hit
GTTTGTGGGTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTT272640.17369790440588329No Hit
ATCGAGGTAATCATCTATCTCAGAGTATCGAAATACTCTCCCCTGGAATC267820.1706271007848579No Hit
CATAATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATACTCTCC266570.16983073055118952No Hit
GAAATACTCTCCCCTGGAATCACTTCAATTTGCTATCCAAAGCTCCCCTA260270.16581702457350073No Hit
AATCACTTCAATTTGCTATCCAAAGCTCCCCTACCGTAGTTTGGGTTTAC252820.16107065798083703No Hit
AGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTATTTAGATTT249680.159070175953862No Hit
CAATTTGCTATCCAAAGCTCCCCTACCGTAGTTTGGGTTTACCGCTTGAG239360.15249534330469564No Hit
AAATACTCTCCCCTGGAATCACTTCAATTTGCTATCCAAAGCTCCCCTAC234460.1493735719887155No Hit
CAGGCATAATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATACT230940.14713099341070526No Hit
CCAATATTGTATCGTCACTGTGGCACTTTTCAGGAATACTAAGACATAGT228450.14554462390523779No Hit
GTCACCATAAAATGGTGCCCCGCCTTATTTTTTCAGCGAGCACAAACACT227040.14464631828165986No Hit
GTGACGTCCCCCTTCTTCCTTAGTCATAACGTAAACTTCACCCTTGAAGT223270.14224446565691592No Hit
AGGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCGCCGTCACC216620.13800777601380002No Hit
GTAATATAATTATCAGGCATAATTATATCGAGGTAATCATCTATCTCAGA216030.13763188926350853No Hit
ATCGTTTCCAATATTGTATCGTCACTGTGGCACTTTTCAGGAATACTAAG215980.1376000344541618No Hit
GTAAAATTCACGTTTACGATATTCTTGCAATGTTCCGTTACGTGAAACTG215920.1375618086829457No Hit
GTAACTTCTGGTAAGTCCTTACCTTCTGAACGGATGTAGTGGTTGTGCTT207400.13213374917026185No Hit
GTTTTCGCGAGCAGCTTCAGCCAAAGCTTGAACACGTCCATGGTAAAGGT202840.1292285905578395No Hit
CTTTTCAGGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCGCC202820.12921584863410082No Hit
GCCGCATCATGGACGAACCCGGAATGGATCTACGCGCGCTACTTGTTAGG199980.1274064954632062No Hit
AGGCATAATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATACTC184010.11723206935785864No Hit
GTTCAATTCGTTGATGTCAGCAGTTAAAACAGCAACTTGAACTTGAGTAG182000.11595150602211986No Hit
CGTCACTGTGGCACTTTTCAGGAATACTAAGACATAGTTAAAACCTTAGT181140.115403603301356No Hit
GTAATCATCTATCTCAGAGTATCGAAATACTCTCCCCTGGAATCACTTCA172210.10971433435202889No Hit
GGGTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCGAA169600.10805151330412927No Hit
GTTAAAACCTTAGTCCGTTTTTCCGCCGTCACCATAAAATGGTGCCCCGC165130.10520369334853104No Hit
GTGTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTATT161630.10297385669425951No Hit
GCCATGTTTTGTCGTAATATAATTATCAGGCATAATTATATCGAGGTAAT157250.10018337539548543No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTGT143250.039.004011
CTCGGAG502.4026285E-630.7357751
CCGTGTC189600.029.6311192
TACGTGA33100.028.5778939
GGAGTCC6650.028.49008835
CGCAACG91650.028.1942211
GGCGTCC21750.026.950581
AAGCCGA58700.026.45245444
GTGGGTT154900.026.4469971
GCCGCAC19200.026.1848688
CGAAGCC60050.026.07236142
GAAGCCG60900.025.88533843
CGGGTCC2050.025.7023892
CCTATCT16250.025.3992313
GTTCTAT8950.025.2656421
ACTCGGT23850.024.91796144
ATCTAGG3300.024.6152291
CGGCAGG14850.024.43987843
CGTGTCC232000.024.4239643
TGGGTTG166150.024.0220262
Produced by FastQC (version 0.11.2)