FastQCFastQC Report
Wed 4 Mar 2015
trimmedLRcomm4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenametrimmedLRcomm4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15289173
Sequences flagged as poor quality0
Sequence length36-50
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTTTTCAGGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCC1542391.0088119220051994No Hit
CCCGTGTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACT1177310.7700285685824865No Hit
CTTCAATTTGCTATCCAAAGCTCCCCTACCGTAGTTTGGGTTTACCGCTT1158930.758006989652089No Hit
GTGGGTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCG828370.5418017050366295No Hit
CCGCGTTCCACCAATATCGTTTCCAATATTGTATCGTCACTGTGGCACTT769910.5035654969696529No Hit
GTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTATTTA604500.3953778271722087No Hit
GTTTTGTCGTAATATAATTATCAGGCATAATTATATCGAGGTAATCATCT589730.38571739622542045No Hit
CGCAACGTTAGGTATGGAAGTGGAATTGTTACTTCAACCTTATACGTCCG568570.37187753713036015No Hit
GCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCGAAGCCGA551060.36042498832343645No Hit
CCGTGTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTA542310.35470198420804055No Hit
GCAACGTTAGGTATGGAAGTGGAATTGTTACTTCAACCTTATACGTCCGG481010.31460825251960983No Hit
GGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCGCCGTCACCA452110.2957059874984736No Hit
CTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCGCCGTCACCATAAAAT426460.27892940972019875No Hit
CAATATTGTATCGTCACTGTGGCACTTTTCAGGAATACTAAGACATAGTT414730.2712573139175023No Hit
GCATAATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATACTCTC398910.26091012247686646No Hit
GTGACGTCCCCCTTCTTCCTTAGTCATAACGTAAACTTCACCCTTGAAGT394380.25794724148912435No Hit
GTCGTAATATAATTATCAGGCATAATTATATCGAGGTAATCATCTATCTC360020.23547382189998112No Hit
CGTGTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTAT345530.22599652708488552No Hit
CAGGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCGCCGTCAC343050.224374464204179No Hit
CAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTATTTAGAT338110.22114341959502976No Hit
CACTTTTCAGGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCG329370.21542695605576576No Hit
ATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATACTCTCCCCTG317960.20796415868928947No Hit
GTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCGAAGC311510.2037454870842262No Hit
GGCATAATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATACTCT309710.20256818338048763No Hit
CACCAATATCGTTTCCAATATTGTATCGTCACTGTGGCACTTTTCAGGAA290760.19017379161057305No Hit
GTTTGTGGGTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTT264370.172913211198539No Hit
GGCACTTTTCAGGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTC250420.16378910749456496No Hit
AAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTATTTAGATT248590.16259218206243073No Hit
CGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCCG244240.15974703144506244No Hit
ATCAGGCATAATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATA243380.15918454189772072No Hit
GCCGCATCATGGACGAACCCGGAATGGATCTACGCGCGCTACTTGTTAGG240590.15735972115692587No Hit
GAAATACTCTCCCCTGGAATCACTTCAATTTGCTATCCAAAGCTCCCCTA236240.15451457053955764No Hit
GTCGTCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCACTAGAGTGCC233810.15292521053951055No Hit
CATAATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATACTCTCC233520.152735533831686No Hit
CTTTAATTCATCATCTTGTCCATCTGTTCCATCATTATCAGATGGGATGA225170.14727415276156533No Hit
GGTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCGAAG224800.14703215144468573No Hit
GCTATCCAAAGCTCCCCTACCGTAGTTTGGGTTTACCGCTTGAGGGGTTT224030.1465285270825309No Hit
GTAAAATTCACGTTTACGATATTCTTGCAATGTTCCGTTACGTGAAACTG222560.1455670623911444No Hit
ATCGAGGTAATCATCTATCTCAGAGTATCGAAATACTCTCCCCTGGAATC212780.13917037893416473No Hit
TAATTATCAGGCATAATTATATCGAGGTAATCATCTATCTCAGAGTATCG209700.13715588148554536No Hit
CAATTTGCTATCCAAAGCTCCCCTACCGTAGTTTGGGTTTACCGCTTGAG207940.1360047400863343No Hit
AACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCCGACTT203600.13316613004509792No Hit
GGGTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCGAA200690.13126282239072054No Hit
CGCGTTCCACCAATATCGTTTCCAATATTGTATCGTCACTGTGGCACTTT191700.1253828444481595No Hit
AGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTATTTAGATTT190140.12436251457158605No Hit
CACCGCGGCATGCTGATCCGCGATTACTAGCGATTCCGACTTCGTGTAGG190040.12429710881026723No Hit
CTTTTCAGGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCGCC177420.11604290173183338No Hit
CAGGCATAATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATACT176700.11557198025033795No Hit
CAAAAACCAATTACGATAGCACATACATTAATCCATGCGAATGTCTTTTG175640.11487867918035856No Hit
CCGCAACGTTAGGTATGGAAGTGGAATTGTTACTTCAACCTTATACGTCC174400.1140676477400053No Hit
CCAATATTGTATCGTCACTGTGGCACTTTTCAGGAATACTAAGACATAGT172860.11306039901569562No Hit
GTCACTGTGGCACTTTTCAGGAATACTAAGACATAGTTAAAACCTTAGTC165290.10810918288386166No Hit
ATCGTTTCCAATATTGTATCGTCACTGTGGCACTTTTCAGGAATACTAAG164600.10765788313076188No Hit
GTCACCATAAAATGGTGCCCCGCCTTATTTTTTCAGCGAGCACAAACACT156090.10209185284253111No Hit
GTGTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTATT153290.10026049152560441No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTGT140100.039.301861
GAGTCTC20250.031.85495643
CCGAGTC20550.030.9947541
AAGCCGA89500.030.9320644
CGAAGCC91250.030.9189542
GAAGCCG91900.030.69981243
GTCGCGG22050.029.4600661
CGGGTCC3050.028.7813222
CGCAACG90900.028.1263031
CGGGAAC38550.028.0085951
CACAGGG22850.027.85302222
GACTCCG4650.027.845163
CGAGTCT23700.027.22626542
TCACAGG23550.027.118421
CCTATGC1900.026.5659183
TACGTGA40050.026.51132839
GGGAACG42050.025.990462
GTTCTAT6850.025.6300851
TCCTATG2000.025.2376232
GGGCGCC17000.025.033344
Produced by FastQC (version 0.11.2)