FastQCFastQC Report
Wed 4 Mar 2015
trimmedLRcomm3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenametrimmedLRcomm3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16855214
Sequences flagged as poor quality0
Sequence length36-50
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTGTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACT1923531.1412076998844394No Hit
CTTCAATTTGCTATCCAAAGCTCCCCTACCGTAGTTTGGGTTTACCGCTT1797681.066542376738735No Hit
GCACTTTTCAGGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCC1309410.7768575350037087No Hit
CGCAACGTTAGGTATGGAAGTGGAATTGTTACTTCAACCTTATACGTCCG1187090.7042865192930805No Hit
CCGCGTTCCACCAATATCGTTTCCAATATTGTATCGTCACTGTGGCACTT897600.5325355109700773No Hit
GTGGGTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCG725420.4303831443492797No Hit
GTTTTGTCGTAATATAATTATCAGGCATAATTATATCGAGGTAATCATCT649710.3854652928168103No Hit
GTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTATTTA601900.35710018276837063No Hit
CTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCGCCGTCACCATAAAAT581790.34516915655891406No Hit
CCGTGTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTA568820.3374742082776285No Hit
CACTTTTCAGGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCG494910.29362427555058035No Hit
GTCGTAATATAATTATCAGGCATAATTATATCGAGGTAATCATCTATCTC482520.2862734344399306No Hit
CAATATTGTATCGTCACTGTGGCACTTTTCAGGAATACTAAGACATAGTT477970.28357397301511567No Hit
CAGGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCGCCGTCAC450960.26754925805154417No Hit
CGTGTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTAT444780.2638827368195978No Hit
CACCAATATCGTTTCCAATATTGTATCGTCACTGTGGCACTTTTCAGGAA440520.2613553289801008No Hit
CAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTATTTAGAT426880.2532628775879084No Hit
CTTTAATTCATCATCTTGTCCATCTGTTCCATCATTATCAGATGGGATGA398790.23659741134108414No Hit
GGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCGCCGTCACCA381650.2264284511605726No Hit
GGCACTTTTCAGGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTC371550.2204362400857088No Hit
CTTTTCAGGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCGCC358170.21249804363207728No Hit
GTTTGTGGGTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTT301030.17859755444220407No Hit
ATCAGGCATAATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATA299230.17752963563678278No Hit
GGCATAATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATACTCT292820.17372665811303256No Hit
GCATAATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATACTCTC280720.16654787058770062No Hit
CAATTTGCTATCCAAAGCTCCCCTACCGTAGTTTGGGTTTACCGCTTGAG276270.16390773798540914No Hit
CCGCAACGTTAGGTATGGAAGTGGAATTGTTACTTCAACCTTATACGTCC253210.1502265115115121No Hit
CCAATATTGTATCGTCACTGTGGCACTTTTCAGGAATACTAAGACATAGT243660.144560608960527No Hit
AATCACTTCAATTTGCTATCCAAAGCTCCCCTACCGTAGTTTGGGTTTAC241240.1431248514554606No Hit
ATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATACTCTCCCCTG238310.14138651695552484No Hit
GTCACCATAAAATGGTGCCCCGCCTTATTTTTTCAGCGAGCACAAACACT234620.13919728340441123No Hit
CTGGAATCACTTCAATTTGCTATCCAAAGCTCCCCTACCGTAGTTTGGGT233020.13824802224403676No Hit
GTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCGAAGC228210.135394305880661No Hit
CAGGCATAATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATACT221750.13156166394564908No Hit
GCAACGTTAGGTATGGAAGTGGAATTGTTACTTCAACCTTATACGTCCGG220550.13084971807536824No Hit
AAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTATTTAGATT219650.1303157586726576No Hit
CATAATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATACTCTCC218760.1297877321521993No Hit
GTTTTCGCGAGCAGCTTCAGCCAAAGCTTGAACACGTCCATGGTAAAGGT214380.1271891297256742No Hit
ATCGTTTCCAATATTGTATCGTCACTGTGGCACTTTTCAGGAATACTAAG211390.12541519793222441No Hit
CAAAAACCAATTACGATAGCACATACATTAATCCATGCGAATGTCTTTTG200900.1191916044495193No Hit
CGCCATTTCAATCTCTACGTTCATATCTCAACTATTTAGATTTCACTAAC197430.11713289430795716No Hit
CTTGTCCAAGTGCAGGACCTACTGGTGGAGCTGGTGTAGCGGCACCGGCA196230.11642094843767631No Hit
AGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTATTTAGATTT187180.11105168999930821No Hit
TAATTATCAGGCATAATTATATCGAGGTAATCATCTATCTCAGAGTATCG184360.10937861720414822No Hit
CGCGTTCCACCAATATCGTTTCCAATATTGTATCGTCACTGTGGCACTTT183980.10915316767855929No Hit
GTGACGTCCCCCTTCTTCCTTAGTCATAACGTAAACTTCACCCTTGAAGT180730.10722498094654866No Hit
GGTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCGAAG179420.10644777337149205No Hit
CAGCAATCTTGTCATGAGTACCTTCAATTGAAGGATCCATTGAAATGCGA172540.10236595038188184No Hit
ATAATTATCAGGCATAATTATATCGAGGTAATCATCTATCTCAGAGTATC171750.10189725268394693No Hit
CTCCTATCTAATATTCGTTTTTTACCAAACGTAAGCGATAACTTCGCCAC170950.10142262210375971No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTGT207450.041.3740121
CGCAACG168350.032.7414471
CGGGTCC2200.031.9294662
CCGTGTC273250.031.4029062
CCTATCT26750.030.1165583
CTATCTA27650.030.0889554
CGTTAGG194700.028.1975966
TCCTATC32000.027.8509732
TAGGTAT203000.026.990629
ACGTTAG209850.026.7267575
AGGTATG206900.026.52429610
CCTATGC7850.026.28583
GGTATGG209500.026.15320211
ACGTCCG214600.026.09326644
CGTGTCC331900.025.98593
TTAGGTA212050.025.8904578
GTATGGA212350.025.83343312
GTTAGGT213200.025.8022867
GTGTCCA335100.025.7181024
TACCGCT326050.025.63338343
Produced by FastQC (version 0.11.2)