FastQCFastQC Report
Wed 4 Mar 2015
trimmedLRcomm2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenametrimmedLRcomm2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17177581
Sequences flagged as poor quality0
Sequence length36-50
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTTTTCAGGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCC1947501.1337451996296801No Hit
CCCGTGTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACT1435780.8358452799611307No Hit
CTTCAATTTGCTATCCAAAGCTCCCCTACCGTAGTTTGGGTTTACCGCTT1288130.7498902202818896No Hit
CCGCGTTCCACCAATATCGTTTCCAATATTGTATCGTCACTGTGGCACTT853420.49682199140845273No Hit
GTGGGTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCG844570.49166992721501357No Hit
CGCAACGTTAGGTATGGAAGTGGAATTGTTACTTCAACCTTATACGTCCG804100.46811014892027003No Hit
GTTTTGTCGTAATATAATTATCAGGCATAATTATATCGAGGTAATCATCT737000.42904760571351697No Hit
GTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTATTTA669430.3897114500580728No Hit
CCGTGTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTA562940.3277178550344195No Hit
GCAACGTTAGGTATGGAAGTGGAATTGTTACTTCAACCTTATACGTCCGG560830.3264895097860403No Hit
GGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCGCCGTCACCA499540.2908092821684264No Hit
GTGACGTCCCCCTTCTTCCTTAGTCATAACGTAAACTTCACCCTTGAAGT494850.28807897922297676No Hit
GCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCGAAGCCGA474450.27620303464149No Hit
GTCGTAATATAATTATCAGGCATAATTATATCGAGGTAATCATCTATCTC463040.2695606558339035No Hit
CTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCGCCGTCACCATAAAAT455490.26516539203046113No Hit
CACTTTTCAGGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCG427370.24879521744068622No Hit
CAATATTGTATCGTCACTGTGGCACTTTTCAGGAATACTAAGACATAGTT409130.23817672581488628No Hit
CAGGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCGCCGTCAC403220.23473619481113203No Hit
CGTGTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTAT401720.23386296359190506No Hit
GCATAATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATACTCTC387240.22543337155563406No Hit
CAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTATTTAGAT375550.21862798958712523No Hit
GGCACTTTTCAGGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTC351820.20481347169895459No Hit
CACCAATATCGTTTCCAATATTGTATCGTCACTGTGGCACTTTTCAGGAA325100.18925831291379153No Hit
GGCATAATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATACTCT323590.18837926015310305No Hit
GTTTGTGGGTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTT311200.18116637028228827No Hit
GTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCGAAGC289860.16874320080341929No Hit
CTTTAATTCATCATCTTGTCCATCTGTTCCATCATTATCAGATGGGATGA287470.16735185239411765No Hit
ATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATACTCTCCCCTG276100.16073275975237725No Hit
ATCAGGCATAATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATA260700.15176758590164705No Hit
AAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTATTTAGATT256310.14921192920004278No Hit
GCCGCATCATGGACGAACCCGGAATGGATCTACGCGCGCTACTTGTTAGG255080.14849587960027666No Hit
CCGCAACGTTAGGTATGGAAGTGGAATTGTTACTTCAACCTTATACGTCC254770.14831541181496974No Hit
TAATTATCAGGCATAATTATATCGAGGTAATCATCTATCTCAGAGTATCG254090.14791954699558688No Hit
GTTTTCGCGAGCAGCTTCAGCCAAAGCTTGAACACGTCCATGGTAAAGGT246200.143326350782453No Hit
CTTTTCAGGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCGCC240370.13993239211039088No Hit
GGTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCGAAG229500.1336043765417261No Hit
CATAATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATACTCTCC224600.13075182122558468No Hit
GTCACCATAAAATGGTGCCCCGCCTTATTTTTTCAGCGAGCACAAACACT224170.1305014949427396No Hit
CAATTTGCTATCCAAAGCTCCCCTACCGTAGTTTGGGTTTACCGCTTGAG221840.12914507578220705No Hit
GAAATACTCTCCCCTGGAATCACTTCAATTTGCTATCCAAAGCTCCCCTA219630.12785851511921265No Hit
AGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTATTTAGATTT203050.1182063993760239No Hit
GGGTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCGAA198120.11533637943549793No Hit
AATCACTTCAATTTGCTATCCAAAGCTCCCCTACCGTAGTTTGGGTTTAC195580.11385770790427359No Hit
CAGGCATAATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATACT192210.11189584843174369No Hit
CGCGTTCCACCAATATCGTTTCCAATATTGTATCGTCACTGTGGCACTTT191690.111593128275745No Hit
GTAAAATTCACGTTTACGATATTCTTGCAATGTTCCGTTACGTGAAACTG187250.10900836386683317No Hit
CCAATATTGTATCGTCACTGTGGCACTTTTCAGGAATACTAAGACATAGT185490.10798377256960687No Hit
CAAAAACCAATTACGATAGCACATACATTAATCCATGCGAATGTCTTTTG183240.10667392574076641No Hit
GTAATATAATTATCAGGCATAATTATATCGAGGTAATCATCTATCTCAGA176640.10283170837616774No Hit
ATCGTTTCCAATATTGTATCGTCACTGTGGCACTTTTCAGGAATACTAAG175490.10216223110809375No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTGT166750.040.4537471
AAGCCGA74750.029.72659544
CGCAACG123700.029.4223161
CCGTGTC233050.029.0110632
GAAGCCG80150.028.01752543
CGAAGCC80200.027.92630842
CCTATGC4800.027.8964773
GACTCCG11650.027.6982383
CGGGTCC3000.027.0733812
TACGTGA31000.026.60226839
TCCTATG5100.026.2555832
CGGAGGC609.929734E-625.61062618
GCGACTC13100.025.4703141
TGACGTC88550.024.9633542
CCTATCT19700.024.1798883
CGTGTCC291150.023.4403823
GTGGGTT174350.023.304861
ACGTCCC95400.023.2628824
GTAAAGG52950.023.22447243
GTGTCCA295150.023.1227074
Produced by FastQC (version 0.11.2)