FastQCFastQC Report
Wed 4 Mar 2015
trimmedLRLB4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenametrimmedLRLB4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15358046
Sequences flagged as poor quality0
Sequence length36-50
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCG950760.6190631282130553No Hit
CCCGTGTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACT917670.5975174185570221No Hit
CTTCAATTTGCTATCCAAAGCTCCCCTACCGTAGTTTGGGTTTACCGCTT864890.5631510675251266No Hit
GCACTTTTCAGGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCC823770.5363768281459764No Hit
CGCAACGTTAGGTATGGAAGTGGAATTGTTACTTCAACCTTATACGTCCG540490.35192628020517713No Hit
GTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTATTTA491420.31997560106279144No Hit
GTTTTGTCGTAATATAATTATCAGGCATAATTATATCGAGGTAATCATCT434600.2829787070568743No Hit
GTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCGAAGC375110.24424331063990823No Hit
CCGCGTTCCACCAATATCGTTTCCAATATTGTATCGTCACTGTGGCACTT365350.23788833553435115No Hit
CTTTAATTCATCATCTTGTCCATCTGTTCCATCATTATCAGATGGGATGA354550.23085619095033313No Hit
GCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCGAAGCCGA352840.22974276805786364No Hit
GTTTGTGGGTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTT335850.2186801628280056No Hit
CCGTGTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTA325950.2122340302926557No Hit
GCAACGTTAGGTATGGAAGTGGAATTGTTACTTCAACCTTATACGTCCGG311500.20282528128903898No Hit
GGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCGCCGTCACCA299960.195311304576116No Hit
AGCAAGTCTACCGACGACTGGCAGATCCTTTAATACCCGTTTACCATGGG294600.19182127726404777No Hit
GGTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCGAAG289590.18855914352646164No Hit
CTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCGCCGTCACCATAAAAT283450.18456123910554767No Hit
GTCGTAATATAATTATCAGGCATAATTATATCGAGGTAATCATCTATCTC280570.18268600054980952No Hit
GCATAATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATACTCTC269800.17567338970074708No Hit
CAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTATTTAGAT264130.17198151379413762No Hit
CAAAAACCAATTACGATAGCACATACATTAATCCATGCGAATGTCTTTTG251080.16348433908844914No Hit
CGTGTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTAT247050.16086030735941279No Hit
GTTCTATTCAGCAAGTCTACCGACGACTGGCAGATCCTTTAATACCCGTT246090.16023522784083338No Hit
CAATATTGTATCGTCACTGTGGCACTTTTCAGGAATACTAAGACATAGTT242760.15806698326076116No Hit
GGCATAATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATACTCT234860.15292309972245166No Hit
ATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATACTCTCCCCTG228250.14861916678723322No Hit
GTGCAATTCAGCAGTATGAGTACCACCACCATAAGGAGTCCGAATGGTAA226320.14736249650508926No Hit
CAGGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCGCCGTCAC224070.14589746638341883No Hit
CAAGACTTCTGCGCCGTTATCACGTAATGTTTGCGCAAAAAGGTCATGTT209980.13672312219926935No Hit
AAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTATTTAGATT204100.13289451014797063No Hit
GTTATTTTCACGATAACCAAAGTAGAATTGGATAGCAGCAATTGCAATGT188950.12302997399538979No Hit
GGGTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCGAA184300.12000224507727089No Hit
ATCAGGCATAATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATA184020.11981993021768524No Hit
CACTTTTCAGGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCG181270.11802933784675472No Hit
AGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTATTTAGATTT173120.11272267318381518No Hit
GCCAAGGTAAGTTACATGAGCACCTTCATCCTTAGCACCAACTTCAAAAG170040.110717209728373No Hit
CACCAATATCGTTTCCAATATTGTATCGTCACTGTGGCACTTTTCAGGAA167480.10905033101216131No Hit
GGCGTCCTTAGCGTCAACAATTACAAGTTGGCCATCCTTCATTTCAGCAA158100.10294278321604194No Hit
GGCACTTTTCAGGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTC155290.1011131233752002No Hit
CATAATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATACTCTCC155260.10109358964024459No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTGT105950.039.236441
GTTCTAT33250.038.1545871
GGAGTCC32900.032.0111635
GAGTCCG34800.030.4770736
TCTATTC40800.030.1792933
AGGAGTC35250.029.91905434
CCGTGTC140550.029.5929992
CTATTCA49000.027.5477034
ATCCTAC9750.025.6630336
CCTCGAG609.941297E-625.6067545
CGCAACG99550.025.44341
AATCGGC30850.024.9723685
CAGTATG43300.024.78735212
GTCCGAA42700.024.77999538
TCGGCGT29350.024.6034247
TTCTATT52400.024.5038972
CGTGTCC179700.023.5486133
GTATGAG45800.023.53048314
ACTCTAG10650.023.08252115
GGCGGCA7350.022.99419616
Produced by FastQC (version 0.11.2)