FastQCFastQC Report
Wed 4 Mar 2015
trimmedLRLB3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenametrimmedLRLB3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13460123
Sequences flagged as poor quality0
Sequence length36-50
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCAATTTGCTATCCAAAGCTCCCCTACCGTAGTTTGGGTTTACCGCTT920430.6838199026858819No Hit
GTGGGTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCG781280.580440461056708No Hit
CCCGTGTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACT722760.5369638895573242No Hit
GCACTTTTCAGGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCC661800.49167455602003046No Hit
CGCAACGTTAGGTATGGAAGTGGAATTGTTACTTCAACCTTATACGTCCG616210.4578041374510471No Hit
CCGCGTTCCACCAATATCGTTTCCAATATTGTATCGTCACTGTGGCACTT424570.3154280239489639No Hit
CTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCGCCGTCACCATAAAAT324790.24129794356262568No Hit
GTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTATTTA317430.23582994003843796No Hit
CTTTAATTCATCATCTTGTCCATCTGTTCCATCATTATCAGATGGGATGA315970.23474525455673773No Hit
GTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCGAAGC314970.2340023192952992No Hit
GTTTTGTCGTAATATAATTATCAGGCATAATTATATCGAGGTAATCATCT288490.21432939357240643No Hit
GGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCGCCGTCACCA260240.19334147243676747No Hit
GTTTGTGGGTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTT251300.1866996311995069No Hit
CAATATTGTATCGTCACTGTGGCACTTTTCAGGAATACTAAGACATAGTT247090.1835718737488506No Hit
CAAGACTTCTGCGCCGTTATCACGTAATGTTTGCGCAAAAAGGTCATGTT246620.18322269417597448No Hit
CAAAAACCAATTACGATAGCACATACATTAATCCATGCGAATGTCTTTTG244370.18155108983773774No Hit
GCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCGAAGCCGA236880.17598650472956304No Hit
GGTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCGAAG231040.17164776280276192No Hit
CCGTGTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTA227240.16882460880929542No Hit
AGCAAGTCTACCGACGACTGGCAGATCCTTTAATACCCGTTTACCATGGG226870.16854972276256316No Hit
CAGGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCGCCGTCAC225700.16768048850668008No Hit
GTGCAATTCAGCAGTATGAGTACCACCACCATAAGGAGTCCGAATGGTAA215350.1599911085507911No Hit
GCATAATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATACTCTC211990.15749484607235759No Hit
GTCGTAATATAATTATCAGGCATAATTATATCGAGGTAATCATCTATCTC211050.15679648692660536No Hit
GCAACGTTAGGTATGGAAGTGGAATTGTTACTTCAACCTTATACGTCCGG202540.15047410785176332No Hit
CAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTATTTAGAT199670.1483418836514347No Hit
ATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATACTCTCCCCTG198120.14719033399620493No Hit
GGCATAATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATACTCT195670.1453701426056805No Hit
CACTTTTCAGGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCG190890.14181891205600425No Hit
CGTGTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTAT185310.13767333329717715No Hit
CACCAATATCGTTTCCAATATTGTATCGTCACTGTGGCACTTTTCAGGAA184670.1371978547298565No Hit
GTTCTATTCAGCAAGTCTACCGACGACTGGCAGATCCTTTAATACCCGTT177600.13194530243148594No Hit
ATCAGGCATAATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATA160330.11911481046644225No Hit
CATCGATGTGTGCGTAGTGACGCTTTTCAGTTTCGTATTCAACGTGGGCA155440.11548185703800776No Hit
CAATTTGCTATCCAAAGCTCCCCTACCGTAGTTTGGGTTTACCGCTTGAG148650.11043732661284002No Hit
CTTCAATCGGCGTATCGCCACTCCGCATCATGATAACATGACCGATTGTA148550.11036303308669616No Hit
GCCAAGGTAAGTTACATGAGCACCTTCATCCTTAGCACCAACTTCAAAAG147930.10990241322460426No Hit
CCAAAAACCAATTACGATAGCACATACATTAATCCATGCGAATGTCTTTT144280.10719069952035358No Hit
ATCGAGGTAATCATCTATCTCAGAGTATCGAAATACTCTCCCCTGGAATC141010.10476130121544952No Hit
CATAATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATACTCTCC139370.1035428873866903No Hit
GTTATTTTCACGATAACCAAAGTAGAATTGGATAGCAGCAATTGCAATGT139340.10352059932884713No Hit
GGCGTCCTTAGCGTCAACAATTACAAGTTGGCCATCCTTCATTTCAGCAA135920.10097976073472732No Hit
ATCGATGTGTGCGTAGTGACGCTTTTCAGTTTCGTATTCAACGTGGGCAG134780.10013281453668736No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTGT81600.039.3059651
GTTCTAT23850.036.3585971
GGAGTCC30850.032.5558135
CCGTGTC103650.031.0501062
AGGAGTC33450.030.1384734
GAGTCCG33700.030.0233736
CGCAACG95650.029.5387441
TCTATTC30500.027.6393743
CCTATGC13450.027.5842533
AATCGGC29700.026.2402975
ATCCTAC8550.025.4194346
CGTATCG28950.025.2518111
CAGTATG41050.025.1356212
TCGGCGT29400.025.0893127
GTATGAG41200.024.99100514
CTATTCA39000.024.4299164
GTCCGAA41350.024.3536338
TCCTATG15750.023.8349342
ATCGGCG32400.023.6470666
ACTCTAG9150.023.51344715
Produced by FastQC (version 0.11.2)