FastQCFastQC Report
Wed 4 Mar 2015
trimmedLRLB2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenametrimmedLRLB2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14229538
Sequences flagged as poor quality0
Sequence length36-50
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCG731290.513923923601736No Hit
CCCGTGTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACT706000.4961510345592387No Hit
GCACTTTTCAGGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCC627710.4411316797495463No Hit
CTTCAATTTGCTATCCAAAGCTCCCCTACCGTAGTTTGGGTTTACCGCTT576600.4052134370068797No Hit
GCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCGAAGCCGA502490.3531316336482604No Hit
CGCAACGTTAGGTATGGAAGTGGAATTGTTACTTCAACCTTATACGTCCG451840.31753666211791276No Hit
CCGCGTTCCACCAATATCGTTTCCAATATTGTATCGTCACTGTGGCACTT347940.2445195339441098No Hit
GCAACGTTAGGTATGGAAGTGGAATTGTTACTTCAACCTTATACGTCCGG321440.22589630106051228No Hit
GTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTATTTA318040.22350690514337151No Hit
CCGTGTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTA290240.20397007970322018No Hit
GTGCAATTCAGCAGTATGAGTACCACCACCATAAGGAGTCCGAATGGTAA288000.20239588945192738No Hit
CAAAAACCAATTACGATAGCACATACATTAATCCATGCGAATGTCTTTTG283350.19912803915348481No Hit
GTTTTGTCGTAATATAATTATCAGGCATAATTATATCGAGGTAATCATCT266270.18712483848737746No Hit
GTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCGAAGC265790.18678751200495758No Hit
GTTTGTGGGTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTT263720.18533279154952184No Hit
AGCAAGTCTACCGACGACTGGCAGATCCTTTAATACCCGTTTACCATGGG259630.18245848881390245No Hit
CTTTAATTCATCATCTTGTCCATCTGTTCCATCATTATCAGATGGGATGA252610.17752508900851172No Hit
GTTCTATTCAGCAAGTCTACCGACGACTGGCAGATCCTTTAATACCCGTT224220.15757363310038597No Hit
GTGACGTCCCCCTTCTTCCTTAGTCATAACGTAAACTTCACCCTTGAAGT215120.15117848520450908No Hit
GGTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCGAAG213460.15001189778614035No Hit
CGTGTCCAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTAT203740.14318103651713782No Hit
CAAGCATATCGCCATTTCAATCTCTACGTTCATATCTCAACTATTTAGAT186550.13110053186547588No Hit
GGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCGCCGTCACCA183570.12900629662045246No Hit
CTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCGCCGTCACCATAAAAT180360.12675042576926954No Hit
GCCAAGGTAAGTTACATGAGCACCTTCATCCTTAGCACCAACTTCAAAAG178500.1254432856498925No Hit
GTCGTAATATAATTATCAGGCATAATTATATCGAGGTAATCATCTATCTC173700.1220700208256937No Hit
GGGTTGCGGCCCTTACGTGCAGCACGTTGACGTACTTCGAAGTTACCGAA173040.12160619691236638No Hit
GTTATTTTCACGATAACCAAAGTAGAATTGGATAGCAGCAATTGCAATGT171370.1204325818589472No Hit
GCATAATTATATCGAGGTAATCATCTATCTCAGAGTATCGAAATACTCTC169220.11892164032310816No Hit
CATCGATGTGTGCGTAGTGACGCTTTTCAGTTTCGTATTCAACGTGGGCA167330.11759341729857989No Hit
CAATATTGTATCGTCACTGTGGCACTTTTCAGGAATACTAAGACATAGTT164130.11534457408244737No Hit
CCAAAAACCAATTACGATAGCACATACATTAATCCATGCGAATGTCTTTT156910.11027062157604836No Hit
GTCGCTGTCAGCATCTTCAAGGTGATCTCCCCAAGTCTTTTCAAGTTCAG156240.1097997700276706No Hit
GTGACTTTTCAAGCAAAACGTCTGTCAAGTCCTGGCCGGCCAAGTTTCCA154660.10868940368970517No Hit
CCGTAGTATACCCAAAAACCAATTACGATAGCACATACATTAATCCATGC154080.10828180085678116No Hit
CAAGACTTCTGCGCCGTTATCACGTAATGTTTGCGCAAAAAGGTCATGTT152840.10741037411052981No Hit
CTTCAATCGGCGTATCGCCACTCCGCATCATGATAACATGACCGATTGTA150610.10584321149428744No Hit
GGGTAACGTTATTTTCACGATAACCAAAGTAGAATTGGATAGCAGCAATT149060.10475392806147325No Hit
CACTTTTCAGGAATACTAAGACATAGTTAAAACCTTAGTCCGTTTTTCCG148870.10462040299551538No Hit
GTGGAATGCAGGAAGACAGTGTTCAAAGAGAACATTAGGGTTTTCAGTAG145560.10229425579382831No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTGT79850.038.8706171
GTTCTAT29350.036.6467061
GGAGTCC42800.030.77813335
GACTCCG8400.029.790143
GAGTCCG44700.029.5916236
AGGAGTC45450.028.96193934
CCGTGTC108850.028.675972
TCTATTC36900.028.5536653
CCTATGC14150.028.5435753
AAGCCGA84100.028.38568544
CGCAACG75750.027.934531
GGGTGTG14200.026.9232742
CGAAGCC90850.026.49666242
CCCTAGG508.4743435E-526.34158922
GTCCGAA51750.025.60647438
GAAGCCG94700.025.3074143
GTATGAG52650.025.30733714
TCCTATG16150.025.28062
CAGTATG54000.025.08106612
CTATTCA44800.025.0374374
Produced by FastQC (version 0.11.2)